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Test Code C AFB BMAR Bone Marrow Culture, AFB

Important Note

Important Note: Bone Marrow sample should be collected in a Wampole Isolator Tube. These tubes can be obtained from SCH Microbiology (7-2585) prior to collecting the sample.

Clinical System Name

Bone Marrow Culture, AFB





Bone Marrow culture for AFB

Bone Marrow culture, Mycobacteria



Includes culture for Acid Fast Bacilli (AFB). Direct Stain is not performed.



If Culture for Bacteria or Yeast is desired, see Bone Marrow Culture.

if Culture for Filamentous Fungi on Bone Marrow is desired, see Bone Marrow Culture, Fungal



Sample Requirements


For detection of AFB, collect using a Wampole ISOLATOR Tube.

Specimen: Bone Marrow

Container: Wampole ISOLATOR Tube

Preferred Vol: 1.5 mL

Minimum Vol: 0.75 mL


Volume      Required- Wampole ISOLATOR Tube
0.75 - 1.5 mL Pediatric ISOLATOR tube


Wampole ISOLATOR Tubes can be obtained by calling the Microbiology Laboratory (x72585).


Note: If provider is requesting culture for both AFB and Filamentous fungi, both cultures can be performed from a single ISOLATOR tube.

Processing Instructions


CPA: Send sample to Microbiology for processing along with the requisition. Micro will prepare paperwork for sendout to UW. Sample must be received at UW within 15 hours of collection. If necessary, sample should be cabbed to UW.


Off-site collection:  Collection of Bone Marrow is unlikely at Off-site locations.




Wampole pediatric ISOLATOR tube
Temp Time
Room temp 15hours
Refrigerated N
Frozen N




Procedure Performed Turn Around Time (TAT)
Bone Marrow Culture, AFB 24/7 6 weeks


Performing Laboratory

University of Washington Microbiology Laboratory

(206) 598-6147




Microbiology Laboratory

CPT Codes



This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (example: MIC's and ID codes)

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

Reporting Information

Negative Final Report: 6 weeks


The report will be updated and the provider will be notified if growth is detected within the given incubation period.