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Test Code C Bone Mar Bone Marrow Culture

Biohazard Infectious

Clinical System Name

Bone Marrow Culture



Bacterial Bone Marrow Culture

Yeast Bone Marrow Culture


Includes culture for Bacteria and Yeast. Direct Stain is not performed.


If Culture for "Filamentous Fungi" on Bone Marrow is desired, see Bone Marrow Culture, Fungal

if Culture for AFB on Bone Marrow is desired, see Bone Marrow Culture, AFB



Sample Requirements

Collection Tube type varies based on the suspected pathogen.


For detection of Bacteria and Yeast, collect in Aerobic Blood culture bottle

Specimen: Bone Marrow

Container: BacT/Alert PF, yellow cap bottle

Preferred Vol: 1.5mL

Minimum Vol: 0.5mL



For detection of AFB and Filamentous Fungi, collect using a Wampole ISOLATOR Tube.

Specimen: Bone Marrow

Container: Wampole ISOLATOR Tube

Preferred Vol: 10 mL

Minimum Vol: 1.0 mL


Volume      Recommended Wampole ISOLATOR Tube
6.0 - 10 mL                            Regular
1.0 - 1.5 mL Pediatric


 Wampole ISOLATOR Tubes can be obtained by calling the Microbiology Laboratory (x72585).

Processing Instructions


CPA: Send sample to Microbiology for processing along with the requisition. 


Off-site collection: Off-site Children's Lab locations should send the sample to SCH Microbiology Laboratory for processing along with the requisition. Sample should be kept at room temperature prior to and during courier transport. 


Other off-site locations, keep sample at room temperature prior to and during courier transport to SCH Microbiology Laboratory.


Aerobic Blood Culture Bottle (BacT/Alert PF, yellow cap)
Temp Time
Room temp 24 hours
Refrigerated N
Frozen N




Procedure Performed Turn Around Time (TAT)
Bone Marrow Culture 24/7 42 days


Performing Laboratory

Seattle Childrens Hospital Laboratory



Microbiology Laboratory

CPT Codes




This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (example: MIC's and ID codes)

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

Reporting Information

Negative Final Report: 42 days


The report will be updated and the provider will be notified if growth is detected within the given incubation period.