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Test Code C Tissue Tissue Culture

Biohazard Infectious

Clinical System Name

Tissue Culture


Includes Bacterial Culture and Gram Stain. Specimen cultured for Aerobic and Anaerobic bacteria.

Sample Requirements

Specimen: Fresh Tissue

Container: Sterile Container, or Amies Transport media from ESwab collection kit.

Preferred Vol: 6 cm3 piece of tissue

Minimum Vol: 0.25 cm3


 Please consult the Photo Swab Guide for more information.

Processing Instructions

CPA: Send sample to Microbiology for processing.  If the sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology.


Regional Clinic collection: Tissue Cultures are not set up at Regional Clinic Lab locations. Samples received at these locations should be kept at room temperature (stable up to 1 hour) and sent via Delivery Express to the Main Campus Microbiology Lab for immediate culture set up. Larger pieces of tissue should be overlayed with Thio broth to keep tissue moist. Small pieces of tissue can be placed in BD ESwab Amies transport media. 


Temp Time
Room temp 1 hour (up to 24 hours is OK if sample is in Amies media)
Refrigerated N
Frozen N



Procedure Performed Turn Around Time (TAT)
Gram Stain 24/7

1 hour

Culture 24/7 48 - 72 hours

Cultures are read at appropriate intervals to detect early bacterial growth. STAT requests are not applicable.

Performing Laboratory

Seattle Children's Laboratory



Microbiology Laboratory

CPT Codes




This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (such as MIC's and ID's)

Reporting Information

Preliminary Report:  18 - 24 hours

Negative Final Report: 48 hours


When Moderate to Abundant Polys are seen on Gram Stain, culture incubation will be extended up to one week.



Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State: