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Test Code C Wound Wound Culture - Deep Wound

Biohazard Infectious

Clinical System Name

Wound Culture


Deep Wound

Anaerobic Culture



Includes Bacterial Culture and Gram Stain. Specimen cultured for Aerobic and Anaerobic bacteria.

Sample Requirements

Specimen: BD ESwab, or syringe

Container: BD ESwab should be inserted into the sterile plastic tube (containing liquid Amies transport media/fluid) provided in the package.

Preferred Vol: N/A

Minimum Vol: N/A


Please consult the Photo Swab Guide to determine which swab to collect.


Main Campus collection: Sample should be collected on a culture swab, or in a sterile syringe (for larger volumes) . The BD ESwab is acceptable for both culture and Gram stain.


Regional Clinic Collection: Samples collected at Regional Clinic locations are optimally collected on a BD ESwab which contains Amies transport media.

Processing Instructions

CPA: Send sample to Microbiology for processing.  If the sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology.


Regional Clinic collection: Samples collected on BD ESwabs are sent to Main Campus Microbiology for culture set up, Swabs can be held at room temperature before and during courier transport. The exception to this includes samples received after the second to the last courier of the day. These late in the day samples should be set up and incubated at the Regional Lab to avoid result delay. Any left over ESwab Amies media can be held at room temp and sent with the plates to SCH Micro.

Also, any fluid/aspirate samples sent in a capped syringe should be set up at the Off-site Labs per Lucidoc instructions. After set up, refrigerate remaining sample and send to Main Campus Microbiology.






BD ESwab
Temp Time
Room temp 24 hours
Refrigerated OK up to 48 hours
Frozen N



Procedure Performed Turn Around Time (TAT)
Gram Stain 24/7

1 hour

Culture 24/7 48 - 72 hours

Cultures are read at appropriate intervals to detect early bacterial growth. STAT requests are not applicable.

Performing Laboratory

Seattle Children's Laboratory



Microbiology Laboratory

CPT Codes




This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (such as MIC's and ID's)

Reporting Information

Preliminary Report:  18 - 24 hours

Negative Final Report: 48 hours


When Moderate to Abundant Polys are seen on Gram Stain and culture is No Growth at 48 hours, the broth culture incubation will be extended for up to one week.



Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State: