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Test Code CCP Anti Cyclic Citrullinated Peptide (CCP)

Clinical System Name

Anti Cyclic Citrullinated Peptide (CCP)


Anti CCP
Anti Cyclic Citrullinated Peptide
CCP Antibody
Cyclic Citrullinated Peptide Antibody
Rheumatoid Arthritis Antibody



This test is used in the evaluation of patients suspected of having juvenile rheumatoid arthritis or other connective tissue diseases that may present with arthritis.  It is used to detect citrullinated antibodies in patient serum or plasma.  Citrullin is an unusual amino acid formed after post-translational modification to arginine. 


Results are obtained using the Bioplex 2200 Anti-CCP IgG assay.  In this method, anti-CCP results > 3 U/mL are considered reactive. Results obtained from other manufacturers’ assay methods should not be used interchangeably.

Sample Requirements

Specimen: Whole Blood

Container(s): Gold SST, Red or Purple EDTA or Gold Microtainer

Preferred Vol: 2.0 mL

Minimum Vol: 1.0 mL



Processing Instructions

Reject due to

Spin: Y

Aliquot: Y

Temp: 2-8 C

Storage location: Spin blood, transfer 1 mL serum to a plastic aliquot tube affixed with a large computer label. Refrigerate in CPA FrigF rack.


Off-site collection: Spin blood, transfer serum to a plastic aliquot container. Ship refrigerated.


Specimen Type Temperature Time
After separation from cells/serum separator Room temp  
  Refrigerated 7 d
  Frozen 1 m



STAT Performed TAT
N M-F 1 - 3 d


Performing Laboratory

Seattle Children's Laboratory


Department: Chemistry 

Location: Chem East

Phone Number: (206) 987-2617

Reference Range


Non-reactive <3.0
Reactive > 3.0


Because of differences between assays from different manufacturers, all results include this comment: "The following results were obtained with the Bioplex 2200 Anti-CCP IgG assay.  Results obtained from other manufacturers' assay methods may not be used interchangeably."


Method: None Immunoassay on Bioplex 2200

Analytical Volume:  0.4 mL


CPT Codes