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Test Code CH50 Cin CH50, Cincinnati

Important Note

This test is used to diagnose and monitor patients with thrombotic microangiopathy (TMA).  All other indications should use CH50 to Mayo Medical Laboratories.

Clinical System Name

CH50, Cincinnati


Total Complement, Cincinnati

Sample Requirements

Specimen: Whole Blood

Container(s): Red Top or Gold SST

Preferred Vol: 2 mL

Minimum Vol: 1 mL


Note: Allow specimen to clot for one hour at room temperature. Separate serum from cells ASAP or within 2 hours of collection.

Processing Instructions

Reject due to:

Spin: Y

Aliquot: Y

Temp: -20

Storage location: Affix large Pathnet label on to aliquot tube and place specimen in Sendouts -20 freezer.


Off-site collection: Send serum frozen.


Specimen Type Temperature Time
Serum Room temp 2 hs
  Refrigerated N
  Frozen 2 wks



STAT Performed TAT
N Mon, Wed, Fri 3-5 d


Performing Laboratory

Cincinnati Children's Hospital

3333 Burnet Ave

Cincinnati, OH 45229


Phone Number: (513) 636-4530


Department: Send Outs


Phone Number: (206) 987-2563

Reference Range

101 - 300 Units



Analytical Volume:


CPT Codes


Send Out Instructions

Reference Test Name: CH50 (Hemolytic Method)
Reference Test Number: CH50 (total hemolytic complement)
Instructions: Ship frozen on dry ice overnight via FedEx to Cincinnati Children's Hospital.