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Test Code CK MB ISO CK Isoenzyme, CK-MB Mass Assay

Clinical System Name

CK Isoenzyme, CK-MB Mass Assay


Following an AMI, the appearance of protein markers in the blood results from necrosis initiated by an ischemic event.  Those proteins that are present in the highest concentrations and those that are most soluble appear in the blood first, e.g., myoglobin.  The structural and mitochondrial proteins of the myocytes appear later following infarction, e.g., CK-MB and proteins of the troponin complex, including troponin I. The simultaneous quantification of myoglobin, CK-MB, and cardiac troponin I following AMI can greatly assist the physician in the management of patients suspected of presenting with an AMI. Other conditions that result in elevated cardiac proteins are:  cardiac contusions, myocarditis, invasive examination of the heart, coronary artery bypass surgery, heart transplant, congestive heart failure and unstable angina.

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 1 mL

Minimum Vol: 1 FULL Lavender/EDTA Microtainers or 0.5 mL EDTA whole blood


Note: If testing cannot be performed immediately or if specimen is collected off site, separate plasma from cells within 4 hours and freeze at -20oC.


Processing Instructions

Reject due to:  Incorrect specimen collection, Insufficient quantity of EDTA whole blood drawn, Age of specimen

Spin: N

Aliquot: N

Temp: 2-8oC

Storage location: Core 5 Chemistry Refrigerator


Off-site collection: Spin blood, separate plasma from cells within 4 hours of collection. Freeze at -20° C.



Specimen Type Temperature Time
EDTA Plasma RT

≤ 4 H

EDTA Plasma 2-8oC

≤ 4 H

EDTA Plasma -20oC or -70oC

≤ 365 D



Specimen Type Temperature Time
EDTA Whole Blood RT

≤ 4 H

EDTA Whole Blood 2-8oC

≤ 4 H



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617

Reference Range

0 - 4.3 ng/mL


Method: Fluorescent Immunoassay (FIA) method by Triage MeterPro and Triage MeterPlus

Analytical Volume: 0.25 mL EDTA  Whole Blood or Plasma

CPT Codes