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Test Code CL Chloride Level

Clinical System Name

Chloride Level


Cl - Blood (Chloride - Blood)


Chloride is increased in dehydration, renal tubular acidosis (hyperchloremia metabolic acidosis), and in excessive infusion of isotonic saline. Chloride is decreased in overhydration, chronic respiratory acidosis, salt-losing nephritis, metabolic alkalosis, and congestive heart failure.

Sample Requirements

Specimen: Whole Blood

Container(s): Gold SST, Dark Green/Sodium Heparin, Red, Gold Microtainer, Lt. Green/Li Heparin Microtainer, or Heparinized Syringe

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note:  Method is affected by EDTA and prolonged exposure to RBCs. Specimen must be spun and aliquot within 4 hours. Not affected by Na, Li or Ammon. heparin.


Processing Instructions

Reject due to: Age of specimen, incorrect specimen collection (No EDTA).

Spin: Y

Aliquot: Y

Temp: 2-8oC

Storage location: Core 5 Chemistry Refrigerator


Off-site collection: Centrifuge sample and remove serum or plasma. Refrigerate at 2-8oC.



Specimen Type Temperature Time
Serum or Plasma RT

7 D

Serum or Plasma 2-8oC

 28 D

Serum or Plasma -20oC or -70oC

365 D



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617


Reference Range


 Age mEq/L
<1 Y 96 - 110
>1 Y 96 - 109



Method: Potentiometric method by Vitros 4600

Analytical Volume: Vitros 4600: 0.05 mL serum or plasma 


CPT Codes


Critical Values

≤ 70 mEq/L

≥ 130 mEq/L