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Test Code CRH CORT CRH Stimulation - Cortisol

Clinical System Name

CRH Stimulation - Cortisol

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold Microtainer, Red

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note: Draw samples at 0, 15, 30, 60, 90 minutes post CRH. Note collection times on requisitions and samples. 


Processing Instructions

Reject due to: gross lipemia

Spin: Y

Aliquot: Y

Temp: 2-8 C

Storage location: CPA refrigerator, FrigD rack. Serum/plasma should be removed from separator for storage.


Off-site collection: Draw samples at 0, 15, 30, 60, 90 minutes post CRH. Note collection times on requisitions and samples. Spin blood. Transfer serum/plasma to plastic aliquot container. Refrigerate.


Specimen Type Temperature Time
gel separator with serum/plasma Refrigerated 48 h
separated serum/plasma Room temp 2 h
  Refrigerated 5 d
  Frozen -20 C 4 w



STAT Performed TAT
N M-F, day shift 1-4 d


Performing Laboratory

Seattle Children's Laboratory    


Department:  Chemistry

Location: Chem West

Phone Number:  (206) 987-2617




Reference Range


Baseline (08:00 A.M.)  4.5 - 23 
Post CRH  2-3 times baseline



Method: Chemiluminescence by Vitros 3600

Analytical Volume: 0.150 mL

Limitations: Turbidity may affect results. Hemolysis will affect results.

CPT Codes