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Test Code Chlam Abs Chlamydia Serology, Serum

Reporting Name

Chlamydia Serology, S

Useful For

Aids in the clinical diagnosis of chlamydial infections


Advisory Information


This test is not intended for medical-legal use.



Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.2 mL


Specimen Type

Serum

Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Day(s) and Time(s) Performed

Monday through Friday; 9 a.m.

Seattle Children's Hospital Note:

TAT: 3 - 6 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Micro-Immunofluorescent Antibody (MIF) Assay

Method Description

The microimmunofluorescent antibody assay is a 2-stage "sandwich" procedure. In the first stage, the patient serum is diluted in phosphate-buffered saline, added to appropriate slide wells in contact with the substrate, and incubated. After incubation, the slide is washed in buffered saline to remove unbound serum antibodies. In the second stage, each antigen well is overlaid with fluorescein-labeled antibody to IgG or IgM. The slide is incubated, allowing antigen-antibody complexes to react with the fluorescein-labeled anti-IgG. After the slide is washed, dried, and mounted, it is examined using fluorescence microscopy. Positive reactions appear as bright apple-green fluorescent elementary bodies with a background matrix of yolk sac. Semiquantitative endpoint titers are obtained by testing serial dilutions of positive specimens.(Schachter J: Chlamydiae [Psittacosis-Lymphogranuloma Venereum-Trachome Group]. In Manual of Clinical Microbiology. Fourth edition. Edited by E Lennette, A Balows, W Hausler, H Shadomy. Washington, DC, ASM Press, 1985, pp 856-861; Smith T: Chlamydia. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections. Sixth edition. Edited by N Schmidt, R Emmons. Washington DC, APHA, 1989, pp 1165-1198)

Reference Values

Chlamydophila pneumoniae

IgG: <1:64

IgM: <1:10

 

Chlamydophila psittaci

IgG: <1:64

IgM: <1:10

 

Chlamydia trachomatis

IgG: <1:64

IgM: <1:10

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86631 x 3-IgG

86632 x 3-IgM

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SCLAM Chlamydia Serology, S 77166-7

 

Result ID Test Result Name Result LOINC Value
185 C. pneumoniae IgG 6913-8
186 C. pneumoniae IgM 6914-6
190 C. trachomatis IgG 6919-5
191 C. trachomatis IgM 6920-3
187 C. psittaci IgG 6916-1
188 C. psittaci IgM 6917-9

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

NA

Testing Algorithm

Includes Chlamydophila pneumoniae, Chlamydophila psittaci, and Chlamydia trachomatis.