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Test Code Chol Cholesterol

Important Note

Patient should be fasting (at least 8 hours). For patients <2 years, fasting is 4 hours.

Clinical System Name


Sample Requirements

Specimen: Whole Blood

Container(s): Gold SST, Red, Dark Green/Sodium Heparin,  Lt. Green/Lithium Heparin Microtainer, or Gold Microtainer

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note:  Method is not affected by hemolysis (hemoglobin <300 mG/dL), icterus (bilirubin <30 mG/dL) or lipemia (triglycerides <200 mG/dL).





Processing Instructions

Reject due to: Age of specimen

Spin: Y

Aliquot: Y

Temp: 2-8oC

Storage location: Core 5 Chemistry Refrigerator


Off-site collection:  Patient should be fasting. Spin blood, refrigerate serum or plasma at 2-8oC. 



Specimen Type Temperature Time
Serum or Plasma RT

Not Recommended   

Serum or Plasma 2-8oC

3 D

Serum or Plasma -20o C or -70oC

21 D



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617

Reference Range


 Age Fasting (mG/dL)
0 - 1 M 50 - 120
> 1 M 125 - 200



Method: Colorimetric method by Vitros 4600

Analytical Volume:  0.02 mL serum or plasma + 0.03 mL dead space

CPT Codes