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Test Code Cytokine Panel Cytokine Panel

Clinical System Name

Cytokine Panel


IL 2

IL 4

IL 6

IL 8

IL 10

IFN gamma

TNF alpha




Quantitative magnetic bead based assay for IL2, IL4, IL6, IL8, IL10, GM-CSF, IFN-gamma, and TNF-alpha. Please contact Chemistry to notify of sample collection when results are needed same day. 

Sample Requirements

Specimen: Whole blood

Container(s):  Lavender/EDTA

Preferred Vol: 2 mL

Minimum Vol:  0.6 mL


Note: Send to Lab within one hour of collection. Heparin is unacceptable.

Processing Instructions

Reject due to:

Spin: Y

Aliquot: Y

Temp: -20 C

Storage location: CRITICAL FROZEN. Separate plasma from cells within 2 hours of collection. Promptly place sample in the CPA freezer, Fz5 rack unless assay will be performed immediately. Call Chemistry (987-2565) when samples are received.


Off-site collection:  CRITICAL FROZEN. Separate plasma from cells within 2 hours of collection. Send frozen.


Specimen Type Temperature Time
Plasma Room temp

1 h

  Refrigerated N

1 y



STAT Performed TAT
N M-F, dayshift 2 - 5 d

Please contact the Chemistry Department (987-2565) if immediate turn around time is necessary.

Weekend samples should be received in lab before 9:00 am.


Department:  Chemistry

Location: Chem East

Phone Number: 206-987-2565

Reference Range


Analyte Reference Interval
Interleukin 2 0 - 9.0 pg/mL
Interleukin 4 0 - 3.0 pg/mL
Interleukin 6 0 - 9.0 pg/mL
Interleukin 8 0 - 116.0 pg/mL
Interleukin 10 0 - 9.2 pg/mL
Granulocyte Macrophage Colony Stimulating Factor 0 - 122.0 pg/mL
Interferon gamma 0 - 124.0 pg/mL
Tumor Necrosis Factor - alpha 0 - 98.0 pg/mL



Method: Quantitative multiplex bead assay using the Bio Rad Bio-Plex

Analytical Volume: 0.1 mL


CPT Codes

83520 x 8

Special Instructions

Please notify the Chemistry  Laboratory (987-2565) of sample collection when same day turn around time is necessary.