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Test Code DIG Digoxin Level

Important Note

No add-ons after 8 hours of collection if specimen is still on gel separator.

Do NOT use green top tubes. Specimen must be serum.

Clinical System Name

Digoxin Level




Digoxin is a widely prescribed cardiac glycoside indicated in the treatment of congestive heart failure and supraventricular arrhythmias. Digoxin measurements are used to monitor patient compliance and therapy, and to diagnose potential overdose.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST, Red Tube, Gold Microtainer

Preferred Vol:  1.0 mL

Minimum Vol:  0.5 mL  


Note:  Draw specimen immediately before next dose (within 30 minutes).  Avoid hemolysis. A hemoglobin level of 300 mG/dl causes a bias of -0.6. Test is also affected if patient is hypertensive, uremic, or a neonate. There is some cross-reactivity to DLIF. Digibind affects method. Method is not affected by moderate lipemia.

Processing Instructions

Reject due to: Hemolysis, age of specimen, incorrect collection container.

Spin: Y

Aliquot: Y

Temp:  2-8oC

Storage location:  Core Chemistry Refrigerator


Off-site collection:  Draw specimen immediately before next dose (within 30 minutes). Avoid hemolysis.

Centrifuge specimens and remove the serum from the cellular material within 4 hours of collection.






Specimen Type Temperature Time
Serum RT

8 H

Serum 2-8oC

7 D

Serum -20o C or -70oC

 4 M



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617



Reference Range

0.5 - 2.0 ng/mL



Method:  Multiple Point Immuno-Rate method by Vitros 4600

Analytical Volume: 0.05 mL serum

MinimumVolume: 0.05 mL serum


Method is affected by EDTA & hemolysis.

CPT Codes


Critical Values

> 2.5 ng/mL