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Test Code Desmgl 1+3 Desmoglein 1 (DSG1) and Desmoglein 3 (DSG3), IgG Antibodies, Serum

Reporting Name

Desmoglein 1 and 3, S

Useful For

Preferred screening test for patients suspected to have an autoimmune blistering disorder of the skin or mucous membranes (pemphigus)


As an aid in the diagnosis of pemphigus

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Specimen Required


Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1 mL

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  36 hours

Reference Values


<14.0 U (negative)

14.0-20.0 U (indeterminate)

>20.0 U (positive)



<9.0 U (negative)

9.0-20.0 U (indeterminate)

>20.0 U (positive)

Day(s) and Time(s) Performed

Once per week, Monday through Friday, day of testing determined by laboratory

Seattle Children's Hospital Note:

TAT: 3 - 12 days

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83516 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DSG13 Desmoglein 1 and 3, S 43309-4


Result ID Test Result Name Result LOINC Value
83680 DSG 1 43311-0
24027 DSG 3 43312-8

Method Description

This enzyme-linked immunosorbent assay (ELISA) method detects and measures serum levels of antibodies of certain pemphigus diseases. Calibrators and patient sera are added to microwells coated with DSG1 and DSG3 antigens, allowing antibodies to react with the immobilized antigens. After washing to remove any unbound serum proteins, horseradish peroxidase conjugated IgG is added and incubated. Following another wash step, the peroxidase substrate is added and allowed to incubate for an additional period. Stop solution is then added to each well to cancel the enzyme reaction and to stabilize the color development. The assay can be quantified by measuring the reaction photometrically and plotting the results.(Ishii K, Amagai M, Hall RP, et al: Characterization of autoantibodies in pemphigus using antigen specific ELISA's with baculovirus expresses recombinant desmogleins. J Immunol 1997 Aug 15;159[4]:2010-2017)

Reject Due To


Mild OK; Gross reject


Mild reject; Gross reject


Mild OK; Gross reject



Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)