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Test Code Estradiol Estradiol

Clinical System Name

Estradiol

Synonyms

Estradiol, Ultra-Sensitive (US)

Estradiol (E2)

Ultrasensitive Estradiol

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Red, Dark Green/Sodium Heparin, Lavendar/EDTA, Gold Microtainer, Lt. Green/ Lithium Heparin Microtainer

Preferred Vol: 2.0 mL

Minimum Vol: 1.0 mL

 

Note: This test requires a minimum of 0.5 mL of serum or plasma for testing.

 

Processing Instructions

Reject due to:

Spin: Y

Aliquot: Y

Temp: -20C

Storage location: CPA freezer, Fz6 rack. Serum/plasma should be removed from separator within 2 hours prior to storage.

 

Off-site collection:  Spin blood. Transfer serum/plasma to plastic aliquot container. Transport specimen frozen.

Stability

Specimen Type Temperature Time
gel separator with serum/plasma Refrigerated 2 h
separated serum/plasma Room temp  
  Refrigerated 14 d
  Frozen -20 C 1 m

 

Availability

STAT Performed TAT
N M, W, F 2-5 d

 

Performing Laboratory

Seattle Children's Laboratory  

Department

Department:  Chemistry

Location: Chem West

Phone Number:  (206) 987-2617

Reference Range

 

Age Estradiol--Female    Age Estradiol--Male 
(Years) (pg/mL)   (Years) (pg/mL)
7 - 9 < 36   7 - 9 < 7
10 - 12 1 - 87   10 - 12  < 11
13 - 15  9 - 249    13 - 15  1 - 36
16 - 17  2 - 266    16 - 17  3 - 34
       >18 10 - 42

 

Methodology

Method: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Analytical Volume: 0.5 mL serum or plasma

Limitations:

CPT Codes

82670