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Test Code FIB Fibrinogen

Clinical System Name

Fibrinogen
 

Synonyms

None specified

Description

The measurement of fibrinogen is important in the evaluation of 1) a patient suffering from uncontrolled bleeding, 2) one who has received large quantities of transfused blood within a short period of time, 3) one who is in danger of developing DIC as a complication of another disease, or 4) one who has demonstrated an unexplained tendency to bleed. In addition to measuring the quantity of normal fibrinogen, this test (which is kinetic) may be used in conjunction with other methods which yield a total clottable fibrinogen level to establish the presence of abnormal or non-functioning fibrinogen.

Sample Requirements

Specimen: Whole Blood

Container(s): Lt Blue/Citrate

Preferred Vol: 1.8 mL

Minimum Vol: N

 

Note:  Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within 1 hour of collection.

Processing Instructions

Reject due to: Clotted, Hemolyzed, Insufficient quantity, and  Improper collection.

Spin: N

Aliquot: N

Temp: RT

Storage location: Core 6 Freezer  ( -70 C).

 

Note: Deliver blood to Core Coag bench.  Coag Technologist will process. Specimen should be centrifuged within 1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label.

 

 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be transferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze one aliquot of 1.0 mL plasma in plastic tube at -70C.

Ship frozen on dry ice.

.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen   -20 C or -70 C 3 m

 

Availability

STAT Performed TAT
Y

Daily

1 h

 

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Coagulation

Phone Number: 206 987-2578

 

 

 

Reference Range

Full Term Infant - Adult       230 - 450 mG/dL

 

 

 

Methodology

Method:  Electromagnetic mechanical clot detection system by STA® - Compact Analyzer

Analytical Volume: 1 mL plasma

This is enough for a PT, PTT, TT, and Fibrinogen on the same sample.

 

Limitations: Method is affected by improper collection, processing and storage.

 

High hematocrit greater than or equal to 56% requires a citrate adjusted tube.

 

Argatroban levels greater than 3ug/mL may falsely decrease Fibrinogen results more than 18%. Argatroban from 2 to 3ug/mL may falsely decrease Fibrinogen results from 10 to 18%. Argatroban levels less than 2ug/mL do not significantly affect Fibrinogen results.

CPT Codes

85384

Critical Values

Critical Value: Less than 60 mg/dL.