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Test Code FLTTKD FLT3-TKD D835/I836 Mutations

Clinical System Name

FLT3-TKD D835/I836 Mutations


FLT3-Tyrosine Kinase Domain Mutations


This assay detects mutations in the FLT3 gene at codons D835/I836 of the tyrosine kinase domain (FLT3 TKD). Evaluation for FLT3 point mutations in the TKD is indicated at diagnosis of acute myeloid leukemia and may provide prognostic information and direct treatment. Positive FLT3 TKD results may be further evaluated by next-generation sequencing to identify the specific mutation upon request. This assay does not detect other point mutations throughout the TKD nor the FLT3 internal tandem duplication (FLT3-ITD).

Sample Requirements

Specimen: Whole Blood or Bone Marrow

Container(s): Lavender/EDTA (preferred) or Dk Green/Sodium Heparin

Preferred Vol: 6 mL blood;1-2 mL bone marrow

Minimum Vol: No microtainers


Note: Record specimen type and date/time of collection on label. While green top tubes are accepted for testing, there is documentation that heparin can interfere with some PCR assays.


Unacceptable:  Formalin fixed, paraffin embedded specimens


For more detailed info for bone marrow testing, see separate Bone Marrow Aspirate listing .

Processing Instructions

Reject due to:

Spin: No

Aliquot: No

Temp: Refrigerate

Notes:  Affix large label to vacutainer. Deliver extra labels and copy of requisition to clear plastic box attached to CPA fridge. Notify sendouts tech so sample can be transported on next available SCCA shuttle. After hours and on weekends, store sample in CPA refrigerated sendouts rack to be sent next weekday.

Storage location: Store sample in CPA refrigerator sendouts rack. 


Off-site collection:  Contact UW Hematopathology at (206) 288-7060 for instructions.


Optimal results are achieved when samples are received within 72 hours of collection for bone marrow and peripheral blood.


Specimen Type Temperature Time
  Room temp 1 day
  Refrigerated 4 days
  Frozen NO



STAT Performed TAT
N Mon - Fri from 0800 - 1500. 2 weeks from day after receipt


Performing Laboratory


UW Molecular Hematopathology Laboratory

825 Eastlake Ave E

Seattle, WA, 98195


Phone Number: (206) 288-7060


Department: Send Outs



Method: PCR amplification of DNA followed by restriction enzyme and capillary electrophoresis for size fractionation

Analytical Volume: None specified

Limitations: None specified

CPT Codes


Special Instructions

Links to:


University of Washington


Place test orders in CIS. For bone marrow, use CIS order "HemOnc Bone Marrow Requisition" or "Hematopoietic Transplant Marrow Requisition", as appropriate.

Send Out Instructions


Reference Test Name: FLT3-TKD D835/I836 Mutations
Reference Test Number: FLTTKD

Send on next available shuttle to UW Molecular Hematopathology 825 Eastlake Ave. If provider desires for same day set up sample must reach UW Hematopath by 830 am. 


Optimal results are achieved when samples are received within 72 hours of collection for bone marrow and peripheral blood.