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Test Code GC PCR Neisseria gonorrhoeae PCR

Biohazard Infectious

Synonyms

Gonorrhoeae PCR

Neisseria gonorrhoeae amplified probe

 

Clincal System Name

Neisseria gonorrhoeae PCR

Description

This test includes nucleic acid amplification for Neisseria gonorrhoeae with amplified probe technique. It is the recommended testing platform for Neisseria gonorrhoeae because of the rapid turn around time and test sensitivity.

If an antibiotic sensitivity is needed, collect a BD E Swab for Genital Culture.

For forensic evaluation of Neisseria gonorrhoeae, please see Sexual Assault Photo Swab Guide and order CT GC PCR and Culture, GC Screen + Wet Mount through the Sexual Assault PowerPlan.

 

Sample Requirements

Specimen: Dirty Catch Urine, Endocervical, Vaginal
Accepted but not validated and not optimal: Urethral, Penile, Rectal, Throat, Eye

Container:

  • Sterile Container for Urines
  • CT/NG Swab Kit (a.k.a. Xpert CT/NG Vaginal/Endocervical Specimen Collection Kit) for all other sites
  • Aptima Multitest Collection Kit for Vaginal, Penile, Throat or Rectal sites (if sample is bloody)
  • Aptima Unisex Colelction Kit for Endocervical or Urethral Sites (if sample is bloody)
  • UTM swabs are acceptable but not optimal and strongly discouraged

Preferred Vol: 10mL for Urine

Minimum Vol: 7mL for Urine

Please consult the Photo Swab Guide to determine which swab to collect.

First-catch urine or dirty catch urine with no genital cleaning (NOT mid-stream clean catch) is required. If the patient has submitted a clean catch sample, a dirty catch sample can be submitted on the following day.

Clean catch urines are unacceptable for testing and will be REJECTED. Bloody swabs/urine samples will be sent to University of Washington for testing.  Send bloody urine to lab in sterile container.  Collect bloody Vaginal or Endocervical samples with Gen-Probe APTIMA collection kit. Call Microbiology (206-987-2585) for APTIMA Kits.

Samples received in Aptima collection kits will be forwarded to the University of Washington for testing.

 

Processing Instructions

CPA: Send sample to Microbiology for processing.

Regional Clinic collection: Send to SCH Main Campus for testing. Refrigerate before and during courier transport. If urine sample received is less than minimal volume, call Microbiology at 206-987-2585 for consult on running or cancelling the test. Additional questions on collection and other specimen sites, contact CF/ Molecular Microbiology Supervisor or Director of Microbiology.

 

Stability

Urine
Temp Time
Room temp 24 hours
Refrigerated 1 week
Frozen N

 

Xpert Vaginal/Endocervical Swabs
Temp Time
Room temp 60 days
Refrigerated 60 days
Frozen N

 

Aptima Multitest Swab Specimen Collection Kit
Temp Time
Room temp 30 days
Refrigerated 30 days
Frozen N

 

Aptima Unisex Swab Specimen Collection Kit
Temp Time
Room temp 30 days
Refrigerated 30 days
Frozen N

Availability

Procedure Performed Turn Around Time (TAT)
In-House PCR (Cepheid) 24/7 3-5 hours
APTIMA PCR (for bloody samples sent to UW for testing) 24/7

3-5 days

Performing Laboratory

Seattle Children's Laboratory

 

Department

Microbiology Laboratory

CPT Codes

87591

Reporting Information

Final Report (Cepheid): 3-5 hours

Final Report (Aptima): 3-5 days

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf