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Test Code HISTONE AB Histone Autoantibodies, Serum

Reporting Name

Histone Autoantibodies, S

Useful For

Evaluating patients suspected of having drug-induced lupus

Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Specimen Type


Specimen Minimum Volume

0.35 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Day(s) and Time(s) Performed

Monday, Wednesday, Friday; 3:00 p.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Method Description

Purified histones are bound to the wells of a polystyrene microwell plate under conditions that will preserve the antigen in its native state. Prediluted controls and diluted patient sera are added to separate wells. After washing, an anti-human IgG conjugated to horseradish peroxidase is added. After incubation and washing, tetramethylbenzidine (TMB) substrate is added to enable visualization of antibodies. The enzyme reaction is stopped and color development measured at 450 nm in a microtiter plate spectrophotometer.(Package insert: QUANTA Lite. Histone ELISA 708520, INOVA Diagnostics, Inc.)

Reference Values

<1.0 Units (negative)

1.0-1.5 Units (borderline)

>1.5 Units (positive)

Units are arbitrarily based on positive control serum.

Reference values apply to all ages.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
HIS Histone Autoantibodies, S 43231-0


Result ID Test Result Name Result LOINC Value
HIS Histone Autoantibodies, S 43231-0

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject


Mild OK; Gross OK