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Test Code HIV Reflex HIV 1/2 Ag Ab with Confirmation

Important Note

Only available in select powerplans.  HIV Ag/Ab with reflex to HIV1 RNA if reactive.

Specimens can not be shared with other testing.

Clinical System Name

HIV 1/2 Ag Ab with Confirmation


This testing detects HIV-1 p24 Antigen in addition to HIV-1 and HIV-2 antibodies. The added detection of HIV-1 p24Ag shortens the antibody-negative window period associated with acute HIV-1 infection. Reactive samples will reflex to a rapid HIV-1/2 discriminatory test (Geenius) for confirmation.


Reactive samples will be sent to UW for HIV 1 RNA testing. 

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 4 mL

Minimum Vol: 3 mL


Note: Other acceptable sample types include: Gold SST, Red, Gold Microtainer, Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer, 

Specimens for this assay cannot not be shared with any other testing.


Processing Instructions

Reject due to:

Spin: Y


Temp: 2 - 8 C

Storage location: Spin blood, refrigerate plasma in a plastic tube affixed with a large computer label in the CPA refrigerator. Store in CPA fridge, FrigF rack


1.5 mL plasma needed to complete all reflex testing


Off-site collection:


Specimen Type Temperature Time
plasma Room temp 48 h
  Refrigerated 7 d
  Frozen 2 m



STAT Performed TAT
N M - F 1-4 d


Please contact the Chemistry Department (987-2565 or 987-3694) if STAT testing is requested.  If Chemistry is unavailable (evenings), contact the clinical pathologist on call.



Performing Laboratory

Seattle Children's Laboratory  


Department:  Chemistry

Location: Chem East

Phone Number:  (206) 987-2617



Reference Range





Method: Immunoassay on Bioplex 2200

Analytical Volume: 1.5 mL plasma needed to for all possible testing


CPT Codes