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Test Code IBD Ser Inflammatory Bowel Disease Serology Panel, Serum

Reporting Name

Inflammatory Bowel Disease Panel, S

Useful For

As an adjunct in the diagnosis of ulcerative colitis and Crohns disease in patients suspected of having inflammatory bowel disease

Profile Information

Test ID Reporting Name Available Separately Always Performed
AASCA Saccharomyces cerevisiae Ab, IgA, S Yes Yes
GASCA Saccharomyces cerevisiae Ab, IgG, S Yes Yes
NSA Neutrophil Specific Antibodies No Yes

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

Saccharomyces cerevisiae ANTIBODY, IgA

Negative: ≤20.0 U

Equivocal: 20.1-24.9 U

Weakly positive: 25.0-34.9 U

Positive: ≥35.0 U

 

Saccharomyces cerevisiae ANTIBODY, IgG

Negative: ≤20.0 U

Equivocal: 20.1-24.9 U

Weakly positive: 25.0-34.9 U

Positive: ≥35.0 U

 

NEUTROPHIL-SPECIFIC ANTIBODIES

Negative (not detectable)

Day(s) and Time(s) Performed

Varies

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions.

CPT Code Information

86255

86671 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IBDP Inflammatory Bowel Disease Panel, S 87551-8

 

Result ID Test Result Name Result LOINC Value
AASCA Saccharomyces cerevisiae Ab, IgA, S 47320-7
GASCA Saccharomyces cerevisiae Ab, IgG, S 47321-5
82966 Neutrophil Specific Antibodies No LOINC Needed

Method Description

IgG antibodies to Saccharomyces cerevisiae antigens (IgG ASCA) are measured by commercial, microtiter enzyme immunoassays (INOVA Diagnostics, San Diego, CA).(3) This assay uses polystyrene microtiter plates coated with partially purified Saccharomyces cerevisiae antigens to capture antibodies from patient's sera and horseradish peroxidase (HRP)-conjugated anti-IgG antibodies to detect IgG ASCA. Results of the test for IgG ASCA are reported in arbitrary units (U).(Package insert: QUANTA LITE ASCA [S. cerevisiae] IgG ELISA 708865)

 

Neutrophil specific antibodies (NSA) are detected qualitatively using an in-house developed method with a substrate of methanol-fixed, human neutrophils and fluorescein conjugated, anti-human IgG antibody as a detection protein. Results of the test for NSA are reported as positive, negative, or indeterminate.(Vidrich A, Lee J, Janes E: Segregation of pANCA antigenic recognition by DNase treatment of neutrophils:  ulcerative colitis, type 1 autoimmune hepatitis, and primary sclerosing cholangitis. J Clin Immunol 1995; Nov15[6]:293-299)

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross OK

Other

NA

Method Name

AASCA, GASCA: Enzyme-Linked Immunosorbent Assay (ELISA)

NSA: Indirect Immunofluorescent Assay (IFA)

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)