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Test Code Insulin Insulin Level

Clinical System Name

 Insulin Level

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Red, Gold Microtainer, Lt. Green/Lithium Heparin

Preferred Vol: 2.0 mL

Minimum Vol: 0.5  mL


Note:  Patients with a history of insulin usage may have insulin antibodies causing  inaccurate results. 

Processing Instructions

Reject due to: any hemolysis, lipemia

Aliquot: Y

Spin: Y

Temp: -20 C

Storage location: CPA freezer,  Fz1 rack

Off-site collection: Spin blood. Transfer serum  to aliquot container. Freeze


Specimen Type Temperature Time
serum/plasma on gel separator Room temp 2 h
separated serum/plasma Room temp 8 h
  Refrigerated 7 d
  Frozen  3 m


Note:Up to a 10% decrease in insulin is seen in the first 2 hrs when centrifuged and left on serum separator. Stable after that up to 8 hrs on SST at room temp.


STAT Performed TAT
N T,Th 2-5 d


Contact Chemistry Lab for request outside of stated availability. (206) 987-2565

Performing Laboratory

Seattle Children's Laboratory    


Department:  Chemistry

Location: Chem East

Phone Number:  (206) 987-2617


Reference Range


Fasting < 17




Method: Chemiluminescence on Siemens Immulite 2000 XPI

Analytical Volume: 0.25 mL serum/plasma

Limitations: Hemolysis affects results

CPT Code