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Test Code Iron Iron (Fe) Level

Clinical System Name

Iron (Fe) Level


Fe (Iron)


The measurements of serum iron and serum iron-binding capacity are useful in the differential diagnosis of anemia, iron deficiency anemia, thalassemia, possible sideroblastic anemia, and iron poisoning.  Serum iron is decreased in cases of insufficient dietary iron, chronic blood loss, inadequate absorption of iron, impaired release of iron stores (commonly observed in inflammation), infection, and chronic diseases.   

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Red Tube, or Dark Green/Sodium Heparin,  Lt. Green/Lithium Heparin Microtainer, or Gold Microtainer

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note:  Only a total iron level will be run in cases of iron ingestion. Method is affected by hemolysis, sodium citrate, EDTA, fluoride, oxalate. Heparin is acceptable for Iron Level, but not TIBC. Not affected by freezing.

Processing Instructions

Reject due to: Age of specimen, incorrect collection container, hemolysis.

Spin: Y

Aliquot: Y

Temp: 2-8oC

Storage location: Core 5 Refrigerator


Off-site collection:  Avoid hemolysis. Spin blood promptly, and store serum at 2-8oC.



Specimen Type Temperature Time
Serum or Plasma RT

 4 D

Serum or Plasma 2-8oC

 7 D

Serum or Plasma -20o C or -70oC

 90 D



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617



Reference Range



Age µg/dL
0 - 1 W 125 - 240
1 W- 12 Y 20 - 123
12 Y- Adult 40 - 140

≥ 350



Method: Two Point Rate by Vitros 4600

Analytical Volume:  0.02 mL serum or plasma + 0.03 mL dead space

CPT Codes