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Test Code Iron Liver Ts Iron, Liver Tissue

Reporting Name

Iron, Liver Ts

Useful For

Diagnosis of hemochromatosis


Advisory Information


This test is for liver tissue only. If tissue is other than liver tissue, see MSCM / Miscellaneous Metals Testing.



Necessary Information


Patient's date of birth is required to calculate iron index.



Specimen Required


Supplies: Metal Free Specimen Vial (T173)

Container/Tube:

Preferred: Mayo metal-free specimen vial (blue label) (T173)

Acceptable: Paraffin block, if not more than 1 or 2 cuts have been made to it for slides.

Specimen Volume: 2 mg

Collection Instructions:

1. Two mg of liver tissue is required. This is typically a piece of tissue from a 22-gauge needle biopsy at least 2 cm long. If an 18-gauge needle is used, the tissue must be at least 1 cm in length.

2. Any specimen vial other than a Mayo metal-free vial used should be plastic, leached with 10% nitric acid for 2 days, rinsed with redistilled water, and dried in clean air.

Additional Information: Paraffin blocks will be returned 3 days after analysis.


Specimen Type

Liver Tissue

Specimen Minimum Volume

2 cm (22-gauge needle), 1 cm (18-gauge needle), or 2 mm x 2 mm (punch) 0.3 mg by dry weight

Specimen Stability Information

Specimen Type Temperature Time
Liver Tissue Refrigerated (preferred)
  Ambient 
  Frozen 

Day(s) and Time(s) Performed

Monday, Wednesday, Friday; 11 a.m.

Seattle Children's Hospital Note:

TAT: 4 - 7 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)

Method Description

After digestion of the liver tissue with nitric acid and hydrogen peroxide, the digest is diluted and tissue iron concentration is analyzed by ICP-MS using Gallium (Ga) as an internal standard and a aqueous acidic calibration.(Unpublished Mayo method)

Reference Values

IRON

Males: 200-2,400 mcg/g dry weight

Females: 400-1,600 mcg/g dry weight

 

IRON INDEX

Reference values have not been established for patients that are <13 years of age.

<1.0 mcmol/g/year (≥13 years)

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83540

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FET Iron, Liver Ts In Process

 

Result ID Test Result Name Result LOINC Value
8350 Iron, Liver Ts 15357-7
7770 Hepatic Iron Index 49061-5

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA

 

Testing Algorithm

See Hereditary Hemochromatosis Algorithm in Special Instructions.

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)