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HelpTest Code LAB1107 Vasoactive Intestinal Polypeptide, Plasma
Additional Codes
VASO INTES
Reporting Name
Vasoactive Intestinal Polypeptide,PSCH Clinical System Name: Vasoactive Intestinal Polypeptide (VIP)
Useful For
Detecting vasoactive intestinal polypeptide-producing tumors in patients with chronic diarrheal diseases
Ordering Guidance
This test should not be requested on patients who have recently received radioactive material.
Specimen Required
Patient Preparation: Fasting (8 hours)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial. Freeze immediately.
Specimen Type
Plasma EDTASpecimen Minimum Volume
0.55 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 90 days |
Day(s) Performed
Monday, Wednesday
TAT: 5 - 10 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Radioimmunoassay (RIA)
Method Description
Vasoactive intestinal polypeptide (VIP) in the patient sample competes with labeled ([125]I) VIP for a limited number of primary antibody binding sites during a 24-hour incubation. Antibody-bound VIP is separated from the unbound portion by a goat anti-rabbit secondary antibody. Centrifugation brings down the heavy antibody complexes while unbound antigen remains in solution and is discarded. The amount of labeled antigen bound to the antibody is inversely proportional to the amount of VIP present in the patient sample. The (125)I signal is counted on a gamma counter and the counts per minute are used to calculate percent of maximum binding and construct a standard curve.(Unpublished Mayo method)
Reference Values
<75 pg/mL
Test Classification
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84586
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| VIP | Vasoactive Intestinal Polypeptide,P | 3125-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8150 | Vasoactive Intestinal Polypeptide,P | 3125-2 |
Report Available
4 to 8 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.