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Test Code LAB2441 Rapid CSF Bact PCR Qual Panel

Important Note

CPA- Send sample with paperwork to Microbiology. CORE: Send sample with paperwork to Microbiology.

This test is only appropriate for pretreated patients or patients with lab verified evidence of meningitis (positive Gram Stain).

Testing will only be performed on CSF from lumbar puncture.  CSF shunt fluid is not an appropriate sample for this testing. 

This test should not replace culture, and should be ordered with a CSF culture on the same sample.

Clinical System Name

Rapid CSF Bact PCR Qual Panel


CSF meningitis, bacterial

Sample Requirements

Specimen: CSF by lumbar puncture

Container:  sterile container

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note: Clotted CSF is not acceptable for this test and will be rejected.

Processing Instructions

CPA: Send CSF to Microbiology ASAP.  The sample can be shared with other Viral PCR tests but cannot be shared or added on to samples that have been run through any analyzer.  Microbiology may split the sample prior to testing if multiple tubes are not collected for Virology and other testing. Please call Microbiology for any sample related questions.


Off-site collection: This test is only available to Seattle Children's Patients. For Seattle Children's Hospital satellite locations, sample should be refrigerated prior to and during transport to the SCH Microbiology Laboratory.  Sample should be frozen at -20C for storage of >24 hours.


Specimen Temp Time
CSF Room temp 24 hours
  Refrigerated 7 days
  Frozen 4 months



Procedure Performed Turn Around Time (TAT)
PCR 24/7

2 hours


Performing Laboratory

Seattle Children's Laboratory


Microbiology Laboratory

CPT Codes




Molecular method by BioFire FilmArray (Film Array)


Appropriate test to diagnose meningitis caused by the following bacteria and yeast: Escherichia coli K1, Listeria monocytogenes, Neisseria meningitidis, Streptococcus agalactiae (Strep B), Streptococccus pneumoniae, Haemophilus influenzae, and Cryptococcus gattii/neoformans.

This test is not appropriate for test of cure or repeat lumbar puncture.

Reporting Information

Final Report: within 2 hours


Positive or negative is reported for each bacteria.

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

Reference Ranges