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HelpTest Code LAB2988 Eculizumab Pharmacokinetic Assay
Additional Codes
EculizPhrm
Clinical System Name
Eculizumab Pharmacokinetic Assay
Sample Requirements
Specimen: Whole Blood
Container(s): Red
Preferred Vol: 4.0 mL
Minimum Vol: 2.0 mL
Note: Place specimen on ice after collection and deliver to lab immediately.
Processing Instructions
Reject due to: Gold SST. Specimens left to clot at 2 - 8 C. Specimens exposed to repeated freeze/thaw cycles. Non-frozen specimens.
Spin: Y
Aliquot: Y
Temp: -20 C
Storage Location: Separate serum from cells within TWO hours of collection. Spin bood and transfer 2.0 mL serum (Min. 1.0 mL) to an aliquot tube affixed with large Epic label. Place sample in the CPA freezer Send Outs rack.
Off-site Collection: Separate serum from cells within TWO hours of collection. Spin bood and transfer 2.0 mL serum (Min. 1.0 mL) to an aliquot tube affixed with large Epic label. Freeze at -20 C. Transport frozen.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Room Temp |
Unacceptable |
| Refrigerated | Unacceptable | |
| Frozen |
> 1 y |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Twice Weekly | 7 - 14 d |
Performing Laboratory
Cincinnati Children’s Hospital
Thrombotic Microangiopathy Laboratory
3333 Burnet Avenue
ATTN: Lab Processing B-4
Cincinnati, OH 45229
Phone: (513) 636-4530
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
80299, 86162
Methodology
Method: ELISA
Analytical Volume: 1.0 mL Serum
Limitations:
Reference Range
| Eculizumab Level | >500 mcg/mL |
| CH50 | 101-300 CH50 Units |
Eculizumab is considered to be present in a therapeutic level in aHUS if the trough serum concentration is >99 μg/mL, and in PNH if the serum concentration is >35 μg/mL.