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Test Code LD Lactate Dehydrogenase

Important Note

This test cannot be ordered as an add-on once it has been stored in fridge.

Clinical System Name

Lactate Dehydrogenase


LDH - Blood


Causes of high lactate dehydrogenase include neoplastic states, hypoxic cardio respiratory diseases, myocardial infarctions, hemolytic anemias, megaloblastic anemias, hepatic cirrhosis, renal infarction, trauma, muscle damage muscular dystrophy, shock, and hypotension.  In myocardial infarction cases, LDH begins to rise within about 12 hours after infarction and usually returns to baseline after 2 to 5 days.

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Red Tube, or Dark Green/Sodium Heparin,  Lt. Green/Lithium Heparin Microtainer, or Gold Microtainer

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note:  Method is affected by oxalate, hemolysis and by exposure to RBC's. A slow freeze and thaw can result in a loss of up to 70% of LDH activity. Not affected by lipemia.


Processing Instructions

Reject due to: Age of specimen, incorrect collection container

Spin: Y

Aliquot: Y

Temp: 2-8oC

Storage location: Core 5 Refrigerator


Off-site collection:  Avoid hemolysis. Spin blood, separate serum/plasma from cells within one hour of collection. Transport specimen at room temperature. Do not refrigerate or freeze. Samples are stable for 2 days at room temperature.



Specimen Type Temperature Time
Serum or Plasma RT

2 D

Serum or Plasma 2-8oC

Not Recommended  

Serum or Plasma -20o C or -70oC

Not Recommended



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617



Reference Range


0- 1 W Up to 2150
1 W- 5 Y 425 - 975
5 - 12 Y 370 - 840
12 - 14 Y 370 - 785
14 - 16 Y 370 - 645
>16 Y 313 - 618



Method: Multiple Point Rate by Vitros 4600

Analytical Volume:  0.02 mL serum or plasma + 0.03 mL dead space

CPT Codes