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Test Code O2 Sat % Oxygen Saturation

Important Note

Avoid air bubbles.

Place specimen in outside pocket of a pre-filled biohazard bag of ice, then put into a separate biohazard bag for transport.

Clinical System Name

% Oxygen Saturation



Oxygen Saturation


Sample Requirements

Specimen:  Whole Blood

Container(s):  Heparinized Syringe, Capillary Blood Gas Tubes

Preferred Vol:  1.0 mL venous blood in a Heparinized Syringe, 2 Capillary Blood Gas Tubes

Minimum Vol: 2 Heparinized Capillary Blood Gas Tubes. (EPOC capillary tubes are not acceptable).  


Note:  Expel air from syringe and cap it immediately after obtaining the sample. Capillary tubes must be filled completely and mixed thoroughly to dissolve and disperse the heparin. EPOC capillary tubes are not acceptable.


Processing Instructions

Deliver to Core Lab. Notify technologist - STAT


Reject due to:  Inadequately filled specimens, air bubbles, visible clots, age of specimen

Spin:  No

Aliquot:  No

Temp: 0-4o C




Specimen Type Temperature Time
Heparinized Syringe, Heparinized Capillary Blood Gas Tube RT ≤ 15 min.
Heparinized Syringe, Heparinized Capillary Blood Gas Tube 0-4o C 30 min
  -20o C or -70oC No



STAT Performed TAT
Yes 24/7 15 minutes


Performing Laboratory

Seattle Children's Laboratory    


Department:  Chemistry - Core

Phone Number: 206-987-2617



Reference Range


Age % Saturation
0 - 1 D 85 - 90
> 1 D

95 - 98



Infant saturation values are slightly lower.


Method:  Oximetry by SIEMENS RAPIDPOINT 405


Analytical Volume: 

0-2 Y  0.2 mL Heparinized Capillary Blood Gas Tube
> 2 Y 0.5 mL Heparinized Syringe


CPT Codes