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Test Code OSTEOCALCN Osteocalcin, Serum

Reporting Name

Osteocalcin, S

Useful For

Monitoring and assessing effectiveness of antiresorptive therapy in patients treated for osteopenia, osteoporosis, Paget's disease, or other disorders in which osteocalcin levels are elevated

 

As an adjunct in the diagnosis of medical conditions associated with increased bone turnover, including Paget's disease, cancer accompanied by bone metastases, primary hyperparathyroidism, and renal osteodystrophy


Specimen Required


Patient Preparation:

1. For 12 hours before this test do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

2. Fasting (12 hours)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL


Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 14 days
  Refrigerated  72 hours

Day(s) and Time(s) Performed

Monday through Friday; 5 a.m.-12 a.m., Saturday; 6 a.m.-6 p.m.

Seattle Children's Hospital Note:

TAT: 3 - 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Electrochemiluminescence Immunoassay

Method Description

Testing is performed using the Roche Cobas 6000 e601. The Roche Osteocalcin assay is a 2-site immunometric (sandwich) assay using electrochemiluminescence detection. Patient specimen, biotinylated monoclonal N-MID osteocalcin-specific antibody, and monoclonal N-MID osteocalcin-specific antibody labeled with ruthenium react to form a complex. Streptavidin-coated microparticles act as the solid phase to which the complex binds. Voltage is applied to the electrode inducing a chemiluminescent emission from the ruthenium, which is then measured against a calibration curve to determine the amount of osteocalcin in the patient specimen.(Package insert: Osteocalcin Roche Cobas. Roche Diagnostics, Indianapolis, IN 2008-2009, V3)

Reference Values

<18 years: not established

≥18 years: 9-42 ng/mL

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83937

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OSCAL Osteocalcin, S 2697-1

 

Result ID Test Result Name Result LOINC Value
OSCAL Osteocalcin, S 2697-1

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

NA

Other

NA