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Test Code PAI-1 Plasminogen Activator Inhibitor Antigen, Plasma

Reporting Name

PAI-1 Ag, P

Useful For

Identification of heredity elevation or deficiency of plasminogen activator inhibitor type 1


Determination of risk for veno-occlusive disease associated with bone marrow transplantation


Differential diagnosis of impaired fibrinolysis


Prognostic marker of occurrence or recurrence of thrombosis

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Plasma Na Cit

Specimen Required

See Coagulation Studies in Special Instructions.


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Spin down, remove plasma, and spin plasma again.

2. Freeze specimen immediately at ≤-40° C, if possible.

Additional Information: 

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Na Cit Frozen 14 days

Special Instructions

Reference Values

3-72 ng/mL

Day(s) and Time(s) Performed


Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
PAI1 PAI-1 Ag, P 22758-7


Result ID Test Result Name Result LOINC Value
86083 PAI-1 Ag, P 22758-7

Method Description

Testing is performed on the Biomek FXP liquid handling system using the Diagnostica Stago, Inc. Asserachrom PAI-1 kit. The method used is an enzyme-linked immunosorbent assay (ELISA). Microtiter plate wells are coated with mouse monoclonal antihuman PAI-1 antibody, which captures the PAI-1 in the sample. Next, a second antibody (mouse monoclonal antihuman PAI-1) coupled with peroxidase binds to another antigenic site distant from the first antibody, forming the “sandwich.’’ The bound enzyme peroxidase is then visualized based on its ability to produce a color reaction when exposed to ortho-phenylenediamine and hydrogen peroxide. The reaction is stopped by the addition of a strong acid (1N HCl). The intensity of the color produced is directly proportional to the PAI-1 concentration in the plasma sample.(Declerck PJ, Alessi MC, Verstreken M, et al: Measurement of plasminogen activator inhibitor 1 in biologic fluids with a murine monoclonal antibody-based enzyme-linked immunosorbent assay. Blood 1988;71[1]:220-225)

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject





Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)