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Test Code PBNP NT-Pro B-Type Natriuretic Peptide (BNP), Serum

Reporting Name

NT-Pro BNP, S
Seattle Children's Hospital Note:

Clinical System Name: NT-Pro B-type Natriuretic Peptide

Useful For

Aids in the diagnosis of congestive heart failure

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Necessary Information


Patient's age and sex are required.



Specimen Required


Patient Preparation: For 12 hours before this test do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.


Seattle Children's Hospital Note:

Draw 2 mL whole blood

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 365 days
  Refrigerated  7 days

Reference Values

Males

≤45 years: 10-51 pg/mL

46 years: 10-53 pg/mL

47 years: 10-55 pg/mL

48 years: 10-56 pg/mL

49 years: 10-58 pg/mL

50 years: 10-59 pg/mL

51 years: 10-61 pg/mL

52 years: 10-62 pg/mL

53 years: 10-64 pg/mL

54 years: 10-67 pg/mL

55 years: 10-68 pg/mL

56 years: 10-70 pg/mL

57 years: 10-71 pg/mL

58 years: 10-73 pg/mL

59 years: 10-76 pg/mL

60 years: 10-77 pg/mL

61 years: 10-79 pg/mL

62 years: 10-82 pg/mL

63 years: 10-83 pg/mL

64 years: 10-85 pg/mL

65 years: 10-88 pg/mL

66 years: 10-89 pg/mL

67 years: 10-92 pg/mL

68 years: 10-95 pg/mL

69 years: 10-97 pg/mL

70 years: 10-100 pg/mL

71 years: 10-103 pg/mL

72 years: 10-104 pg/mL

73 years: 10-107 pg/mL

74 years: 10-110 pg/mL

75 years: 10-113 pg/mL

76 years: 10-116 pg/mL

77 years: 10-119 pg/mL

78 years: 10-122 pg/mL

79 years: 10-125 pg/mL

80 years: 10-128 pg/mL

81 years: 10-131 pg/mL

82 years: 10-135 pg/mL

≥83 years: 10-138 pg/mL

 

Females

≤46 years: 10-140 pg/mL

47 years: 10-141 pg/mL

48 years: 10-144 pg/mL

49 years: 10-146 pg/mL

50 years: 10-149 pg/mL

51 years: 10-150 pg/mL

52 years: 10-152 pg/mL

53 years: 10-155 pg/mL

54 years: 10-157 pg/mL

55 years: 10-160 pg/mL

56 years: 10-162 pg/mL

57 years: 10-166 pg/mL

58 years: 10-168 pg/mL

59 years: 10-171 pg/mL

60 years: 10-173 pg/mL

61 years: 10-177 pg/mL

62 years: 10-179 pg/mL

63 years: 10-183 pg/mL

64 years: 10-185 pg/mL

65 years: 10-189 pg/mL

66 years: 10-193 pg/mL

67 years: 10-196 pg/mL

68 years: 10-199 pg/mL

69 years: 10-202 pg/mL

70 years: 10-206 pg/mL

71 years: 10-210 pg/mL

72 years: 10-214 pg/mL

73 years: 10-218 pg/mL

74 years: 10-222 pg/mL

75 years: 10-227 pg/mL

76 years: 10-230 pg/mL

77 years: 10-235 pg/mL

78 years: 10-239 pg/mL

79 years: 10-244 pg/mL

80 years: 10-248 pg/mL

81 years: 10-253 pg/mL

82 years: 10-258 pg/mL

≥83 years: 10-263 pg/mL

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Seattle Children's Hospital Note:

TAT: 3 days

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83880

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PBNP NT-Pro BNP, S 33762-6

 

Result ID Test Result Name Result LOINC Value
PBNP NT-Pro BNP, S 33762-6

Method Description

This is an automated, double-incubation sandwich assay. In the first incubation, antigen from the patient specimen reacts with biotinylated monoclonal sheep N-terminal pro brain natriuretic peptide (NT-proBNP) antibody and monoclonal NT-proBNP antibody labeled with ruthenium complex. During the second incubation, streptavidin-labeled microparticles are added, and the resulting complex is bound to the solid-phase via biotin-streptavidin interaction. The resulting reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of an electrode. Unbound substances are washed away. Voltage is then applied to the electrode, which induces chemiluminescent emission that is measured by a photomultiplier. Results are obtained by comparing this measurement against the calibration curve. The Roche E Modular NT-proBNP assay standardization is traceable to an internal Roche standard. Assigned values for calibrators are traceable to this standardization.

 

The Roche N-terminal pro brain natriuretic peptide (NT-proBNP) assay is automated and more precise than the Biosite BNP assay used previously. In addition, in vitro NT-proBNP is more stable than BNP.(Package insert: ProBNP II, Roche Diagnostics Corporation, Indianapolis IN)

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

NA

 

Method Name

Electrochemiluminescence Immunoassay

Forms

If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/cardiovascular-request-form.pdf).

Testing Algorithm

See Laboratory Approach to the Diagnosis of Amyloidosis in Special Instructions.