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Test Code PNEUMO Streptococcus pneumoniae IgG Antibodies, 23 Serotypes, Serum

Reporting Name

S. pneumoniae IgG Ab,23 serotypes,S

Useful For

Assessing the IgG antibody response to active immunization with nonconjugated, 23-valent vaccines

 

Assessing the IgG antibody response to active immunization with conjugated, 13-valent vaccines

 

Determining the ability of an individual to produce an antibody response to polysaccharide antigen(s), as part of an evaluation for humoral or combined immunodeficiencies


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in Red Top.

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Day(s) and Time(s) Performed

Monday through Friday; 8 a.m. 

Seattle Children's Hospital Note:

TAT: 6 - 8 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Microsphere Photometry

Method Description

IgG antibodies to Streptococcus pneumoniae serotypes are measured by microsphere photometry. Purified pneumococcal polysaccharides coupled covalently to polystyrene microspheres bind IgG antibodies in patients' sera during the first incubation. After incubation, the microspheres are washed and incubated with phycoerythrin-conjugated antihuman IgG antibody. The concentration of IgG antibodies to each polysaccharide is determined by comparison to dose-response curves calculated from serial dilutions of a serum pool from immunized adults with known concentrations of antibodies to each polysaccharide (secondary standard). The secondary standard is traceable to a standard reference preparation (FDA 89-SF) that contains known concentrations of IgG antibodies to 23 different Streptococcus pneumoniae serotypes. Dose-response curves prepared from serial dilutions of the secondary standard parallel the dose-response curves of the primary reference preparation for all polysaccharides.(Jacob GL, Homburger HA: Simultaneous Quantitative Measurement of IgG Antibodies to Streptococcus Pneumoniae Serotypes by Microsphere Photometry. Poster Presentation; AAAAI 60th Annual Meeting, March 19-23, 2004. San Francisco, CA. J Allergy Clin Immunol Vol 113, No 2, [Abstract 1049, pS288] 2004; Plikaytis BD, Holder PF, Pais LB, et al: Determination of parallelism and nonparallelism in bioassay dilution curves. J Clin Microbiol 1994;2441-2447; Plikaytis BD, Goldblatt D, Frasch CE, et al: An analytical model applied to a multicenter pneumococcal enzyme-linked immunosorbent assay study. J Clin Microbiol 2000;38:2043-2050)

Reference Values

Results are reported in mcg/mL. 

Serotype

Normal Value

1 (1)

 ≥2.3

2 (2)

 ≥1.0

3 (3)

 ≥1.8

4 (4)

 ≥0.6

5 (5)

 ≥10.7

8 (8)

 ≥2.9

9N (9)

 ≥9.2

12F (12)

 ≥0.6

14 (14)

 ≥7.0

17F (17)

 ≥7.8

19F (19)

 ≥15.0

20 (20)

 ≥1.3

22F (22)

 ≥7.2

23F (23)

 ≥8.0

6B (26)

 ≥4.7

10A (34)

 ≥2.9

11A (43)

 ≥2.4

7F (51)

 ≥3.2

15B (54)

 ≥3.3

18C (56)

 ≥3.3

19A (57)

 ≥17.1

9V (68)

 ≥2.6

33F (70)

 ≥1.7

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86317 x 23

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PN23 S. pneumoniae IgG Ab,23 serotypes,S 42366-5

 

Result ID Test Result Name Result LOINC Value
23979 Serotype 1 (1) 85954-6
23949 Serotype 2(2) 86039-5
23950 Serotype 3 (3) 86080-9
23951 Serotype 4 (4) 86107-0
23952 Serotype 5 (5) 86130-2
23953 Serotype 8 (8) 86147-6
23954 Serotype 9N (9) 86169-0
23955 Serotype 12F (12) 85977-7
23956 Serotype 14 (14) 85991-8
23957 Serotype 17F (17) 86009-8
23958 Serotype 19F (19) 86024-7
23959 Serotype 20 (20) 86045-2
23960 Serotype 22F (22) 86052-8
23961 Serotype 23F (23) 86064-3
23962 Serotype 6B (26) 27118-9
23963 Serotype 10A (34) 86098-1
23964 Serotype 11A (43) 86122-9
23965 Serotype 7F (51) 25296-5
23966 Serotype 15B (54) 40973-0
23967 Serotype 18C (56) 27395-3
23968 Serotype 19A (57) 40974-8
23969 Serotype 9V (68) 30153-1
23970 Serotype 33F (70) 40969-8

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross OK

Other

NA

Forms

If not ordering electronically, complete, print, and send a General Request Form (T239) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/general-request-form.pdf).