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Test Code PRF GRNZ B Perforin/Granzyme B Analysis

Important Note

Samples should be drawn Monday - Thursday 10AM - 1330 PM ONLY.

Clinical System Name

Perforin/Granzyme B Analysis

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender Top/EDTA

Preferred Vol: 3 mL

Minimum Vol: 1 mL


Notes: Samples should be drawn Monday - Thursday 10AM - 1330 PM ONLY.   Samples drawn outside of these times will be rejected.  Send tubes to lab immediately. Whole blood samples MUST reach the reference lab within 24 hours of the blood draw.  May share WHOLE BLOOD sample with IL-2R.


Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: RT

Storage location: Affix large Cerner labels to tubes and place in room temp send-outs rack. Store extra labels and copy of requisition in the box attached to CPA refrigerator. Alert Send-outs team. 


Off-site collection: No off-site collection.


Specimen Type Temperature Time
Whole blood Room temp 24 h



N 3-5 d


Performing Laboratory

Cincinnati Children’s Diagnostic Immunology Laboratories


Department:  Cincinnati Children’s Diagnostic Immunology Laboratories, 3333 Burnet Ave, Cincinnati, OH 45229

Phone Number: (513) 636-4685

Reference Range

Interpretive report is provided.


Method: In this assay, peripheral blood is stained with both surface and intracellular monoclonal antibodies and analyzed using four-color flow cytometry.

Analytical Volume:


CPT Codes

CPT code

Send Out Instructions

Reference Test Name: Perforin/Granzyme B
Reference Test Number: None specified.
Instructions: Send FedEX PRIORITY Overnight to Cincinnati Children's at room temperature.