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Test Code Syphilis Scr Syphilis Screen

Important Note

Neonates (<30 days old) should be screened with RPR, per CDC recommendations.

Clinical System Name

Syphilis Screen



T. pallidum antibody




This test has replaced the RPR Screen for diagnosis of syphilis.


Syphilis Screen is a treponemal testing procedure for the qualitative detection of antibodies to T. pallidum to aid in the diagnosis of syphilis.  Positive results are sent to both Washington State Laboratory and Mayo Medical Laboratories for confirmation with RPR titer.

Sample Requirements

Specimen: Whole Blood

Container(s): Gold SST, Red

Preferred Vol: 3.0 mL

Minimum Vol: 1.0 mL


Note: *3 mL is required to have enough specimen to send for both confirmation tests.

Processing Instructions

Reject due to: gross hemolysis or gross hemolysis

Spin: Y

Aliquot: Y

Temp: -20 C

Storage location: Spin blood, transfer 1.5 mL serum to a plastic aliquot tube affixed with a large computer label. Freeze in CPA Freezer, Fz1 rack.


Off-site collection: Spin blood, transfer serum to a plastic aliqout container. Ship frozen.


Specimen Type Temperature Time
After separation from cells/serum separator Room temp 2 h
  Refrigerated 3 d
  Frozen 14 d



STAT Performed TAT
N T, Th 2 - 5 d


For requests outside of stated availability, please contact the Chemistry Lab (206-987-2565).

Performing Laboratory

Seattle Children's Laboratory


Department: Chemistry 

Location: Chem West

Phone Number: (206) 987-2617

Reference Range





Method: None Chemiluminscence on Siemens Immulite

Analytical Volume:  0.2 mL

Limitations: gross lipemia or gross hemolysis is not acceptable

CPT Codes