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Test Code TBILI GRP Bilirubin Total

Clinical System Name

Bilirubin Total Group for SCCA and RSH




Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST, Red, or Dark Green/Sodium Heparin, 1 Gold, Lt. Green/Lithium Heparin, or Red Microtainer

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note:   Avoid hemolysis.  Protect sample from light.  In-patients:  wrap paper towel around tube and seal with one piece of tape.  Off-site:  wrap tube in foil.  Specimen stable under fluorescent lighting for up to 4 hours. This test is only available for Seattle Cancer Care Alliance and research patients. The test includes Bilirubin Unconjugated, Conjugated, Delta & Total. Method is affected by exposure to UV light, gross hemolysis and lipemia (very slight lipemia OK).




Processing Instructions

Reject due to: Marked hemolysis, gross lipemia, prolonged light/UV exposure, age of specimen

Spin: Y

Aliquot: Y

Temp: 2-8o C

Storage location:  CORE 5 Refrigerator



Off-site collection: Avoid hemolysis. PROTECT FROM UV LIGHT (wrap specimen in foil). Spin blood, refrigerate serum or plasma. Specimen stable under fluorescent lighting up to 4 hours.


Specimen Type Temperature Time
Plasma or Serum RT

≤ 4 H

Plasma or Serum 2-8oC

≤ 7 D

Plasma or Serum -20oC or -70oC

≤ 180 D



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Lab Chemistry

Phone Number: 206-987-2617



Reference Range



Unconjugated (BU)

Conjugated (BC)

Total bili  Delta bili (calculated)

Neonate (0 - 1 month)

0.6 - 10.5 mg/dL

0.0 - 0.6 mg/dL

2 weeks- adult     0.1-1.3  mg/dL 0.0-0.2 mg/dL

1 month - adult

0.0 - 1.1 mg/dL

0.0 - 0.3 mg/dL


Critical level for neonates up to one month

≥ 20.0 mg/dL

≥ 20.0 mg/dL

≥ 20.0 mg/dL






Method: Colorimetric by Vitros 4600

Analytical Volume:  22 uL + 30 uL dead space

CPT Codes

82248, 82247, 84311

Critical Values



0-1 M

≥ 20.0 mg/dL