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Test Code UA Urinalysis

Clinical System Name

Urinalysis

Description

Urinalysis with reflex Microscopic if indicated.

Sample Requirements

Specimen: Urine

Container(s): Sterile, screw-capped container preferred. Clean container also acceptable.

Preferred Vol:  12.0 mL fresh random urine

Minimum Vol: 2.0 mL

 

Notes: Specimen must be received in the Lab within two hours. If there will be a delay, keep the specimen refrigerated (up to 10 hours) and transport on ice. Do not use cotton balls or gauze to collect specimen. Do not squeeze urine from diapers.

For bagged urines: Aspirate urine sample from bag using a sterile needleless syringe and place in sterile specimen cup before sending to the lab.

For non-sterile containers: Urines collected in non-sterile containers will not be sent for culture. They will have the following comment appended: “Urine collected into a non-sterile container. Results may be affected due to unknown interferents, and sample is unsuitable for culture. For future collections please collect urine into a sterile specimen cup.”

 

UA testing cannot be performed off 24 hour urine collections.

 

Acceptable collection methods: bagged, bladder tap, bladder wash, clean catch, cystoscopy, direct cath, foley cath, lleal loop, indwelling cath, kidney, midstream void, nephrostomy, suprapubic aspirate, ureter. The urinalysis includes the biochemical tests listed in the reference range and specific gravity.

Processing Instructions

Reject due to:  Insufficient quantity, incorrect storage temperature, age of specimen, gross contamination, specimens collected with cotton balls or gauze, specimens obtained from diapers, specimens sent in any preservative solution.

Spin: N

Aliquot: N

Temp: RT

Storage location: Deliver to Core Lab Urinalysis bench. 

 

Off-site collection:  Deliver specimen to the Lab within two hours of collection or keep refrigerated at 2-8oC.

Stability

 

Specimen Type Temperature Time
Urine RT

 2 H

Urine 2-8oC

 10 hrs

Urine -20oC or -70oC

N/A

 

Availability

STAT Performed TAT
Y 24/7  1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Urinalysis

Phone Number: 206-987-2617

Reference Range

 

Biochemical tests

Reference Values

Leukocyte Esterase

Negative

Specific Gravity (Random Specimen)

1.001 - 1.035

pH

4.5 - 8.0

Qualitative Protein

Negative

Glucose

Negative

Ketones (Acetone)

Negative

Bilirubin

Negative

Blood (Hemoglobin)

Negative

Reducing Substances

Negative

Nitrites

Negative

Urobilinogen

Less than or equal to 1.0 mg/dL.

 

 

Microscopic Results

 

RBCs

Up to 5/hpf.

WBCs

Up to 5/hpf.

Crystals

Most are diet dependent.

Casts - hyaline

May be present, relative to clinical picture.

Casts - non-hyaline

None seen.

Bacteria

None to few. Presence is relative to specimen collection.

Epithelial Cells

None to few. More than a few can indicate non-clean collection.

 

Methodology

Method:  Clinitek 500 Urine Analyzer with Seimens Reagent strips

 

Analytical Volume: 2.0 mL

CPT Codes

81001