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Test Code Vit D Fra Vitamin D, 25-Hydroxy Fractionated

Clinical System Name

Vitamin D, 25-Hydroxy Fractionated


25-Hydroxyvitamin D

25-OH Vitamin D



Vitamin D2

Vitamin D3

Vitamin D Level, Nutritional


Useful in the nutritional assessment for Vitamin D status, specifically for patients supplemented with Vitamin D2 (this is not common). For general screening, and for those patients supplemented with Vitamin D3, consider ordering Vitamin D, 25-Hydroxy Level which provides a total level.

Sample Requirements

Specimen: Whole Blood

Container(s): Gold SST, Red, Dark Green/Sodium Heparin, Lavendar/EDTA, Gold Microtainer, Lt. Green/ Lithium Heparin Microtainer

Preferred Vol: 1.5 mL

Minimum Vol: 1 mL




Processing Instructions

Reject due to:

Spin: Y

Aliquot: Y

Temp: 2- 8 C

Storage location: Transfer 0.7 mL serum to plastic tube. Deliver to CPA freezer, Fz7 rack. 


Off-site collection:  Allow blood to clot for serum; spin blood and refrigerate serum.  Frozen serum is also acceptable.


Specimen Type Temperature Time
serum or plasma Room temp 2 h
  Refrigerated 7 d
  Frozen 1 m



STAT Performed TAT
N T 2 - 7 days


Performing Laboratory

Seattle Children's Laboratory    



Department:  Chemistry

Location: Chem West

Phone Number: 206-987-2617




Reference Range


Vitamin D sufficiency ≥ 30 ng/mL
Vitamin D insufficiency 15 - 30 ng/mL
Vitamin D deficiency < 15 ng/mL


Note: Cord blood levels tend to be lower (30 - 50%) in the US. Levels of Vitamin D vary with exposure to sunlight, peaking in the summer in ambulatory individuals. 


Method: LC/MS/MS

Analytical Volume: 0.5 mL serum or plasma


CPT Codes