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Test Code WTLOH Wilm's Tumor Loss of Heterozygosity

Important Note

Tumor tissue AND a normal sample is required.


Tumor sample collected during or after the treatment (e.g. post-chemotherapy) CANNOT be accepted. However, pre-treatment relapse tumor samples will be accepted

Additional Codes

Wilm's Tumor LOH

Clinical System Name

Wilm's Tumor Loss of Heterozygosity


Wilm's Tumor LOH

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 4 mL

Minimum Vol:




Specimen: Tumor

Container(s):Sterile Screw-Capped Container

Preferred Vol: Snap‐frozen tumor tissue is preferred.

Alternative: OCT‐embedded or paraffinembedded tumor is also accepted.


Note: Please note, at least 40% tumor (based on internal pathology review) must
be present in the submitted sample.

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: Refrigerate whole blood and immediately freeze tumor

Storage location: Deliver blood to Sendouts refrigerator and deliver Tumor to Sendouts -70


Off-site collection:


Specimen Type Temperature Time
Whole Blood Room temp 24 h
  Refrigerated 72 h
  Frozen N
Tumor Room Temp N
  Refrigerated N
  Frozen 24 h



STAT Performed TAT
N Monday to Friday 3 wks


Performing Laboratory

Nationwide Children's Hospital


700 Children's Drive

Columbus, OH 43205


Department: ChildLab at Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205

Phone Number: (614) 722-5321

Reference Range

Interpretive report is provided



Analytical Volume:


Special Instructions

Nationwide ChildLab

Lab Requisition



*Normal/control sample required

Send Out Instructions

Reference Test Name: Wilms Tumor Loss of Heterozygosity (LOH)
Reference Test Number: WTLOH

Ship whole blood overnight, ambient temperature. Ship tumor on dry ice. 


Nationwide Children's Hospital
ChildLab Central Processing Room C‐1961
Attn: Cytogenetics/Molecular Genetics
700 Children’s Drive
Columbus, OH 43205