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Lipid Laden Macrophages

Important Note

Note: The stain performed for this test is an Oil Red O stain

Clinical System Name

Cytology Final Report

Synonyms

Macrophages, Lipid-Laden

Oil Red O Stain

Bronchoalveolar lavage, histo/path

Description

Oil-red-O stain applied to air dried fluid preparation is microscopically reviewed by pathologist to semiquantitate intracellular lipid.

Sample Requirements

Specimen:  Body Fluid

Container(s):  Sterile Specimen Container, Sterile Screw-Capped Tube or Syringe (BAL)

Preferred Vol:  1.0 mL - 2.0 mL BAL or other body fluid

Minimum Vol:  none specified (see BAL requisition)

 

Note: Acceptable specimens: Bronchoalveolar lavage or Bronchial washing. Send fluid with BAL requisition to Laboratory immediately, no fixative. BAL samples from different sites will be pooled for all tests unless otherwise specified. Non-BAL samples will be processed accordingly.

Processing Instructions

Reject due to: NA

Spin:  N

Aliquot:  N

Note:  Deliver specimen and copy of requisition to Core Hematology.  Notify tech immediately.  For Non-BAL samples make 4 unstained cytopreps and deliver to Histology with copy of requisition. 

 

Storage location:  For BAL, refer to BAL requisition for instructions. BAL specimens are often split between departments; ensure shared specimens remain sterile. Cytocentrifuge preparations for pathology are made in Microbiology.

 

Off-site collection: Send fluid with BAL requisition to Laboratory immediately, no fixative.

 For Non-BAL samples make 4 unstained cytopreps ASAP after specimen collection.  Do not fix slides.

Stability

Specimen Type Temperature Time
Body Fluid Room temp 10 min
  Refrigerated 30 min
  Frozen N

 

Availability

STAT Performed TAT
  M-F 8 a.m. - 5 p.m. 1 day

 

Performing Laboratory

Seattle Children's Laboratory  

Department

Department:  Histology

Phone Number: (206) 987-2580; for results inquiries call (206) 987-2103.

 

 

Methodology

Method: Oil-red- O stain on unfixed cytocentrifuge preparation

Analytical Volume:  n/a

CPT Codes

CPT codes will vary depending on tests performed.  Call Client Services for more information (206) 987-2617.