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Test Code CMVU97 CMV Drug Resistance UL97

Clinical System Name

CMV drug resistance UL97

Synonyms

Ganciclovir Resistance Test
UL97 sequencing

 

Description

For CMV PCR positive patients. UL97 for gancyclovir resistance. The UL54 test, for foscarnet/cidofovir resistance, is a send out test through CHRMC send out dept.

This test can be added onto a positive CMV DNA PCR Qual or Quant test, if sample still available (regardless of sample type).

Sample Requirements

Specimen: Whole Blood or CSF

Container(s): Lavender/EDTA or CSF Tube

Preferred Vol: 3.0 mL whole blood or 1.0 mL CSF

Minimum Vol:  1.0 mL whole blood or 0.5 mL CSF

 

Note:

Processing Instructions

Reject due to: Green top

Spin: Y

Aliquot: Y

Temp: -20 C

Storage location: Blood: Centrifuge within 24 hours of collection & aliquot serum/plasma. Deliver to CPA freezer.

CSF: Freeze within 2 hours of collection or ship immediately @ 4°C. If CSF has cells, centrifuge & aliquot clear CSF. Deliver to CPA freezer.

Monday – Friday: Place sample in the Virology Section of the Sendouts Freezer Rack.

Saturday-Sunday & Holidays: Close batch in Mayo Access and courier to UW NW220. Place a copy of the Transfer List (indicate time sent) in the Virology Transport Binder. (Call on Holidays to be sure the lab is open to accept samples).

 

Off-site collection: Blood: Centrifuge tube within 2 hours of collection & aliquot serum/plasma - Freeze @ -20°C
CSF: Freeze within 2 hours of collection or ship immediately @ 4°C. If CSF has cells, spin & aliquot clear CSF-Freeze @ -20°C.

Stability

Specimen Type Temperature Time
  Room temp

 

  Refrigerated  
  Frozen

 

 

Availability

STAT Performed TAT
N Collected daily, performed on M 3- 7 d

 

Performing Laboratory

University of Washington

Dept of Laboratory Medicine

UW Molecular Virology Lab
1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

 

Department:  Send Outs
 

Phone Number: (206) 987-2563

Reference Range

None specified
 

 

Methodology

Method: DNA Sequencing

Analytical Volume: None specified

Limitations:

CPT Codes

87910

 

 

 

 

 

 

 

 

 

Send Out Instructions

 

Reference Test Name: CMV Drug Resistance, Rapid UL97
Reference Test Number:

CMVU97

Instructions:

Send out M - F with the UW courier. Weekends and Holidays courier on dry ice to UW NW220.