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Test Code HRTABB HIV 1 RNA Quantitation

Clinical System Name

HIV 1 RNA Quantitation

Synonyms

HIV-1 RNA by RT PCR
HIV-Q RNA QUANT, PCR

HRTPCR

Sample Requirements

Specimen: Whole Blood or CSF

Container(s): Lavender/EDTA or Sterile Screw-Capped Container

Preferred Vol: 7 mL Whole Blood or 3 mL CSF

Minimum Vol: 4 mL Whole Blood or 2 mL CSF

 

Note:  Specimens for this assay should not be shared with any other testing.

Processing Instructions

Reject due to: Serum, ACD (yellow top), and heparin (green top) are unacceptable.

Spin: Y

Aliquot: Y

Temp:-70 C

Storage location: Spin blood and remove plasma within 6 hours of collection. Transfer 2 - 3 mL plasma or CSF to a plastic aliquot tube. Freeze specimen in the CPA -70 C freezer send-outs rack.

Specimens for this assay should not be shared with any other testing.

 

Off-site collection: Spin blood and remove plasma within 6 hours of collection. Transfer 2 - 3 mL plasma or CSF to a plastic aliquot tube. Transport CSF or plasma frozen.

Stability

Specimen Type Temperature Time
EDTA Whole Blood Room temp 6 h
  Refrigerated 24 h
Plasma Room temp 24 h
  Refrigerated 5 d
CSF Refrigerated 5 d

 

Availability

STAT Performed TAT
N

Drawn daily; Set up M - F at HMC Retrovirus Lab

4 - 7 d

 

Performing Laboratory

University of Washington

Dept. of Laboratory Medicine

Harborview Medical Center Retrovirus Lab

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department:  Send Outs

 

Phone Number: (206) 987-2563

 

 

Reference Range

None specified

 

Methodology

Method: RealTime RT PCR

Analytical Volume: 2.0 mL plasma or CSF

Limitations:

CPT Codes

87536

 

Send Out Instructions

Reference Test Name:

HIV1 RNA Quantitation

Reference Test Number:

HRTABB

Instructions:

Send out M - F with the UW courier.