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Muscle Biopsy, Histo/Path

Important Note

Muscle biopsies should be scheduled between 0700 and 1500, Mon - Fri and must be performed on SCH main campus. Muscle biopsies can be ordered in CIS; clinical "details" must be complete.

See sample requirements  below for detailed instructions.

Clinical System Name

Surgical Pathology Final Report

Sample Requirements

Specimen:  Tissue

Container(s):  see notes

Preferred Vol:  n/a

Minimum Vol:  n/a

 

Notes: 

A 1 x 0.5 x 0.5 cm sample dissected from the belly of the muscle along the longitudinal plane should be obtained with minimal trauma and no cautery.  Sample should then be gently stretched and sutured to a moistened tongue blade to maintain fiber orientation.  Sample should be IMMEDIATELY transported to pathology OC.8.901 on saline-moistened Telfa pad accompanied by a completed surgical pathology requisition form.  Do not flood with excess saline, do not cool with ice.  If Mitochondrial enzyme testing has been requested, a second piece of similar-sized muscle is required.  The 2nd piece may be in direct continuity with the first or may be submitted separately on a moist saline Telfa pad.

Processing Instructions

Reject due to: NA

Spin:  N

Aliquot:  N

Temp: 

Note:  Notify Histology immediately when specimen arrives in Lab.  Contact on-call pathologist if Histology is unavailable.

 

Off-site collection:  Not available

Stability

Specimen Type Temperature Time
Tissue Room temp 10 min
  Refrigerated N
  Frozen N

 

Availability

STAT Performed TAT
N M-F 8 a.m. - 5 p.m. 4 weeks

 

Performing Laboratory

Seattle Children's Laboratory  

Department

Department:  Histology

Phone Number: (206) 987-2580; for results inquiries call (206) 987-2103.

 

 

CPT Codes

CPT codes will vary depending on tests performed.  Call Client Services for more information (206) 987-2617.

Methodology

Method: Routine histology, enzyme histochemical analysis and electron microscopic examination (send out); additional special testing dependent upon clinical indications.

Requisition

Surgical pathology requisition required.

Description

 Comprehensive evaluation of muscle biopsy specimens, including enzyme histochemical analysis and electron microscopic examination send out to assess neurologic and musculoskeletal disorders.