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HelpLipid Laden Macrophages
Clinical System Name
Cytology Final Report
Synonyms
Macrophages, Lipid-Laden
Oil Red O Stain
Bronchoalveolar lavage, histo/path
Sample Requirements
Specimen: Body Fluid
Container(s): Sterile Specimen Container, Sterile Screw-Capped Tube or Syringe (BAL)
Preferred Vol: 1.0 mL - 2.0 mL BAL or other body fluid
Minimum Vol: none specified (see BAL requisition)
Note: Acceptable specimens: Bronchoalveolar lavage or Bronchial washing. Send fluid with BAL requisition to Laboratory immediately, no fixative. BAL samples from different sites will be pooled for all tests unless otherwise specified. Non-BAL samples will be processed accordingly.
Processing Instructions
Reject due to: NA
Spin: N
Aliquot: N
Note: Deliver specimen to Core Hematology. Notify tech immediately. For Non-BAL samples make 4 unstained cytopreps and deliver to Histology.
Storage location: For BAL, refer to BAL requisition for instructions. BAL specimens are often split between departments; ensure shared specimens remain sterile. Cytocentrifuge preparations for pathology are made in Microbiology.
Off-site collection: Send fluid with BAL requisition to Laboratory immediately, no fixative.
For Non-BAL samples make 4 unstained cytopreps ASAP after specimen collection. Do not fix slides.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Body Fluid | Room temp | 10 min |
| Refrigerated | 30 min | |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| M-F 8 a.m. - 5 p.m. | 1 day |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Histology
Phone Number: (206) 987-2580; for results inquiries call (206) 987-2103.
CPT Codes
CPT codes will vary depending on tests performed. Call Client Services for more information (206) 987-2617.
Methodology
Method: Oil-red- O stain on unfixed cytocentrifuge preparation
Analytical Volume: n/a
Description
Oil-red-O stain applied to air dried fluid preparation is microscopically reviewed by pathologist to semiquantitate intracellular lipid.