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HelpBotulism - Culture & Toxin
Clinical System Name
Miscellaneous Test
Synonyms
Botulism
Bot toxin
C. botulinum
Clostridium Botulinum Culture
Clostridium Botulinum Toxin
Red toxin bioassay
Sample Requirements
Specimen: Stool
Container(s): Sterile Screw-Capped Container
Preferred Vol: 50.0 g
Minimum Vol: 15.0 g
Note: Specimen requirements: 10.0 g (walnut-sized)- 50.0 g (ping-pong ball sized) stool in a sealed, leakproof, sterile container or enema with sterile water or saline not to exceed 20.0 mL. Deliver to the lab ASAP. Method is affected by age of sample or improper storage or shipping.
Processing Instructions
Reject due to:
Spin: N
Aliquot: N
Temp: 2 - 4 C
Storage Location: Affix a large label to the sterile container and refrigerate in the CPA refrigerator.
Off-site Collection: Ordering physician must get approval from State Epidimiologist before State Lab will accept the sample. Refrigerate sample. Transport refrigerated.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Stool | Room Temp | |
| Refrigerated | Y | |
| Frozen |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y (24/7 for critical siutations only) | Daily | 4 - 14 d |
Performing Laboratory
Washington Public Health Laboratories (State Lab)
1610 NE 150th Street
Shoreline, WA 98155
Phone Number: (206) 418-5400
Department
Department: Send Outs
Phone Number: (206) 987-2563
Methodology
Method: Real-Time Polymerase Chain Reaction (RT-PCR), Mouse bioassay, ELISA, standard reference procedures for biochemical identification
Analytical Volume: 10.0 g Stool
Limitations:
Reference Range
None specified.
Send Out Instructions
| Reference Test Name: | Clostridium botulinum toxin |
| Reference Test Number: | N/A |
| Instructions: | Send sample to the State Microbiology Lab within 24 hours of collection (excluding weekends). Send specimen on an ice pack via Delivery Express. Call the State Lab with any questions (206) 418-5400. |