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Body Fluid Malignancy Workup - Flow Cytometry

Important Note

Specimens for Fluid Malignancy Workup must be coordinated with Pathologist on-call and pathologist on cytology prior to collection to ensure testing availability and adequate specimen collection.  Contact pathologyadministrationintake@seattlechildrens.org or 72103 to determine who the pathologists are.  In Epic, order a “Miscellaneous test”.  Under name of test put “flow cytometry at SCH”, and make sure to choose the correct specimen type (CSF, body fluid, and other are choices) and specimen source (BAL, CSF, and other are choices). Under specimen details, note to collect an additional (ideally) 10-20 mL for flow.  Under Handling and Storage, note “send to Cell Markers immediately”.

For further information, contact Hematopathology (Cell Markers) at 206-987-2560 or Pathology 987-2103.

Note:  'Flow Cytometry FHCRC' is only to be used for BMT/HSCT patients.  For all other SCH patients, order Miscellaneous test (flow cytometry).

Clinical System Name

Miscellaneous test request in Epic; Cell Marker Final Report

Synonyms

Body Fluid Flow Workup Request
Flow Cytometry Immunophenotyping
Immunophenotyping
Malignancy, Body Fluid

Sample Requirements

Specimen:  Body Fluid (specify type)

Container(s):  sterile collection tube/container

Preferred Vol: 10-20 mL; volume dependent on blast count 

Minimum Vol:  dependent on blast count; consult lab

Note:  Specimens for Body Fluid Malignancy Workup must be coordinated with Pathologist on-call and pathologist on cytology prior to collection to ensure testing availability and adequate specimen collection.

For further information, contact Hematopathology (Cell Markers) at 206-987-2560 or Pathology 987-2103. 

Note: this test is for Body Fluid workups only - for bone marrow, peripheral blood, or CSF, see separate listing(s).

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  N

Aliquot:  N

Temp:  RT

Storage location:  Days: M - F until 1630, transport specimen and labels to the Cell Markers Lab (station #181). Call before tubing specimen to make sure there is someone available to do testing.

After hours: Contact pathologist on call.

Off-site collection:  not accepted

Stability

Temperature Time
Room temp must be processed immediately
Refrigerated N
Frozen N

Availability

STAT Performed TAT
Y - with pathologist approval

Monday - Friday 0800-1630;  SEE ORDERING NOTES ABOVE.

 2-3 days

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

CPT Codes

contact Laboratory Client Services at (206)-987-2617

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on blast count; consult lab

Limitations:  None specified

Reference Range

None specified
 

Description

Body Fluid Malignancy Workup (by Flow Cytometry) is used in determining the lineage of hematopoietic precursors which may be present in body fluids in various hematopoietic disease states.

Requisition

Order online in Epic -- Miscellaneous test.  Specify flow cytometry.