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CSF Malignancy Workup - Flow Cytometry

Important Note

Specimens for CSF Malignancy Workup must be coordinated with Hematopathologist on cytology service prior to collection to ensure testing availability and adequate specimen collection.  Contact pathologyadministrationintake@seattlechildrens.org or 72103 to determine who the appropriate hematopathologist is. This flow cytometry order can only be placed by the hematopathologist as a reflex test after cytology review. Please make sure that there is a Cytology order in Epic (as well as a cell count, if indicated). Under specimen details, note to collect an additional (ideally) 5-10 mL for flow. 

 

Note:  'Flow Cytometry FHCRC' is only to be used for BMT/HSCT patients.  For all other SCH patients, please order a Cytology specimen and contact the hematopathologist as above.

Clinical System Name

Miscellaneous Test

Synonyms

CSF Flow Workup Request
Flow Cytometry Immunophenotyping (Leukemia)
Immunophenotyping
Malignancy, CSF

MRD

Description

CSF Malignancy Workup (by Flow Cytometry) is used in determining the lineage of hematopoietic precursors which may be present in the CSF in acute leukemia.

Sample Requirements

Specimen:  Cerebrospinal Fluid (CSF)

Container(s):  CSF collection tubes

Preferred Vol:  5 - 10 mL

Minimum Vol:  dependent on blast count; consult lab

Note:  Note: change to Specimens for CSF Malignancy Workup must be coordinated with Hematopathologist on cytology service prior to collection to ensure testing availability and adequate specimen collection.  Contact pathologyadministrationintake@seattlechildrens.org or 72103 to determine who the appropriate hematopathologist is. This flow cytometry order can only be placed by the hematopathologist as a reflex test after cytology review. Please make sure that there is a Cytology order in Epic (as well as a cell count, if indicated).

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  N

Aliquot:  N

Temp:  RT

Storage location:  Days: M - F until 1630, transport specimen and labels to the Cell Markers Lab (station #181). Call before tubing specimen to make sure there is someone available to do testing.

After hours: Contact pathologist on call.

Off-site collection:  not accepted

Stability

Temperature Time
Room temp must be processed immediately
Refrigerated N
Frozen N

Availability

STAT Performed TAT
Y - with pathologist approval

Monday - Friday 0800-1630;  SEE ORDERING NOTES ABOVE.

 2-3 days

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on blast count; consult lab

Reference Range

None specified
 

CPT Codes

contact Laboratory Client Services at (206)-987-2617

Requisition

Order online in Epic -- Miscellaneous test.  Specify flow cytometry.