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Th17 Enumeration

Important Note

Collect Monday through Thursday 1000 AM - 1400 PM. No off-site collections.

Additional Codes

For Research Use Only (RUO) testing, see "Hyper IgE Screen".

Clinical System Name

Miscellaneous Test

Synonyms

T helper 17

Hyper IgE Syndrome (Th17)

Sample Requirements

Specimen: Whole Blood

Container(s): Dark Green/Sodium Heparin

Preferred Vol: 3 mL

Minimum Vol: 2 mL

 

Note: Samples must be drawn Monday through Thursday, 1000 AM and 1400 PM ONLY. Samples drawn outside of these times may be rejected. If drawn outside of the times, contact the Send Outs department at x72563 for further clarification. Send sample(s) to Main Lab immediately after collection. Specimens are stable for only 24 hours at room temperature.

Processing Instructions

Reject due to: Frozen, clotted, hemolyzed or centrifuged specimens. Specimen collected in an unacceptable anticoagulant.

Spin: N

Aliquot: N

Temp: RT

Storage Location: Affix a large Cerner label to the sample(s), place in the room temperature Send Outs rack, and notify the Send Outs department

 

Off-site collection: No off-site collection.

Stability

Specimen Type Temperature Time
  Room temp 24 h
  Refrigerated Unacceptable
  Frozen Unacceptable

 

Availability

STAT Performed TAT
N T - F 2 - 4 d

 

Performing Laboratory

Cincinnati Children's Hospital

CCHMC - Julie Beach

Diagnostic Immunology Laboratory, Rm R2328

3333 Burnet Avenue

Cincinnati, OH 45229-3039

 

Phone Number: (513) 636-4685

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

88184, 88185 (x4), 88187

Methodology

Method: Flow Cytometry

Analytical Volume: 2 mL Sodium Heparin Whole Blood

Limitations:

Send Out Instructions

Reference Test Name Th17 Enumeration
Reference Test Code 7372838
Instructions Ship ambient, Monday through Friday, via FedEx Priority Overnight. Saturday delivery is not acceptable.

 

Description

Tests Included: Cell Surface expression of CCR6+CD45RA- intracellular expression of IL17A+ on CD4+ Lymphocytes, Population of Th17 cells among Total CD4+ T cells

Reference Ranges

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