Home
HelpHeme Gene Panel by NGS
Clinical System Name
Miscellaneous Genetic Test
Description
This assay is a multiplexed gene sequencing panel designed to detect mutations associated with hematologic myeloid and lymphoid disorders. Purified genomic DNA is enzymatically sheared and targeted sequences are isolated by hybrid capture probes and sequenced by using an Illumina NGS platform.
This assay will not detect variants if present in only a small proportion of cells in the sample. If indicated, additional testing for FLT3-ITD and CEBPA mutations for new evaluation of acute myeloid leukemia should be separately ordered.
This assay is not intended for post-treatment disease monitoring nor comprehensive germline evaluation and is not validated to detect all classes of genomic alterations, such as large structural events including fusions and copy alterations.
Single gene testing can also be ordered as a MISC genetic test.
Sample Requirements
Specimen: Bone Marrow
Container(s): Lavender/EDTA
Preferred Vol: 2.0 mL
Minimum Vol: 1.0 mL
Specimen: Blood
Container(s): Lavender/EDTA
Preferred Vol: 6.0 mL
Processing Instructions
Reject due to:
Spin: N
Aliquot: N
Temp: RT or 2 - 4 C (see Storage Location instructions)
Storage Location: During business hours, call Send Outs for special handling. If received after hours or on weekends, place sample in the CPA 1 refrigerator's Send Outs rack.
Optimal results are achieved when samples are received within 48 hours of collection for bone marrow and peripheral blood.
Stability
| Specimen Type | Temperature | Time |
| Bone Marrow | Room temp | 3 d |
| Refrigerated | 7 d | |
| Frozen |
Availability
| STAT | Performed | TAT |
| N | Weekly | 10 - 12 d |
Performing Laboratory
UW / Seattle Cancer Care Alliance
Hematopathology Laboratory
825 Eastlake Avenue E., G7-800
Seattle, WA 98109
Phone Number: (206) 288-7060
Intake Hours: Monday - Friday 08:00 - 18:30 PST
Department
Department: Send Outs
Phone Number: (206) 987-2563
Reference Ranges
Interpretive report provided.
Methodology
Method: Amplification of the stated genetic regions followed by sequencing using an Illumina instrument
Analytical Volume: 1.0 mL Bone Marrow
Limitations:
Send Out Instructions
| Reference Test Name: |
Heme Gene Panel by NGS |
| Reference Test Code: | HCAPA |
| Instructions: | During business hours, call Send Outs for special handling. If received after hours or on weekends, place sample in the CPA 3 refrigerator's Send Outs rack. Send Monday through Friday via Delivery Express. |