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Pitted RBC Percent

Important Note

Notify the Send Outs department prior to collection of this test. Send Outs can be contacted at ext. 7-2563 or by email at referencelabteam@seattlechildrens.org.

 

Samples should be drawn Monday through Thursday 08:00 AM - 14:00 PM ONLY. Please contact the Send Outs department during business hours if planning to collect outside of these times.

Clinical System Name

Miscellaneous Test

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 2.0 mL

Minimum Vol: 1.0 mL

 

Note: Collect in Lavender/EDTA vacutainer and place on ice immediately. Send to Main Lab ASAP. Whole blood must be aliquoted within TWO hours of collection. Aliquot 100 uL of fresh blood into 0.5 mL of 2% Glutaraldehyde (available from Histology, ext. 7-2580).

Processing Instructions

Reject due to: Clotted samples; RBCs that have not been placed in 2% glutaraldehyde solution within TWO hours post collection.

Spin: N

Aliquot: N

Temp: 2 - 4 C

Storage Location: See note below.

Note: Collect in Lavender/EDTA vacutainer and place on ice immediately. Send to Main Lab ASAP. Aliquot 100 uL of fresh blood into 0.5 mL of 2% Glutaraldehyde (available from Histology, ext. 7-2580) into a Falcon tube. Once aliquoted, place the aliquot into the CPA 3 refrigerator's Send Outs rack.

 

Off-site Collection: DO NOT collect at any off-site locations; including Seattle Children's Regional Labs.

Stability

Specimen Type Temperature Time
Whole Blood in 2% Glutaraldehyde Room Temp Unacceptable
  Refrigerated 90 d
  Frozen Unacceptable

 

Availability

STAT Performed TAT
N Daily 1 w

 

Performing Laboratory

Children's Medical Center

1935 Medical District Drive

Dallas, TX 75235

 

Phone Number: (214) 456-2320

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

85999

Methodology

Method: Interference Phase Microscopy (Nomarki Optics)

Analytical Volume: 100 uL Whole Blood in 0.5 mL 2% Glutaraldehyde

Limitations:

Send Out Instructions

Reference Test Name: Pitted RBC Percent
Reference Test Code:  
Instructions: Collect in Lavender/EDTA vacutainer and place on ice immediately. Send to Main Lab ASAP. Whole blood must be aliquoted within TWO hours of collection. Aliquot 100 uL of fresh blood into 0.5 mL of 2% Glutaraldehyde (available from Histology, ext. 7-2580).

 

Reference Ranges

See individual components.